A direct comparison of next generation sequencing enrichment methods using an aortopathy gene panel- clinical diagnostics perspective
- Whitney L Wooderchak-Donahue†1,
- Brendan O’Fallon†1,
- Larissa V Furtado2,
- Jacob D Durtschi1,
- Parker Plant1,
- Perry G Ridge1,
- Alan F Rope3,
- Angela T Yetman4 and
- Pinar Bayrak-Toydemir1, 2, 5Email author
© Wooderchak-Donahue et al.; licensee BioMed Central Ltd. 2012
Received: 18 July 2012
Accepted: 9 October 2012
Published: 14 November 2012
Aortopathies are a group of disorders characterized by aneurysms, dilation, and tortuosity of the aorta. Because of the phenotypic overlap and genetic heterogeneity of diseases featuring aortopathy, molecular testing is often required for timely and correct diagnosis of affected individuals. In this setting next generation sequencing (NGS) offers several advantages over traditional molecular techniques.
The purpose of our study was to compare NGS enrichment methods for a clinical assay targeting the nine genes known to be associated with aortopathy. RainDance emulsion PCR and SureSelect RNA-bait hybridization capture enrichment methods were directly compared by enriching DNA from eight samples. Enriched samples were barcoded, pooled, and sequenced on the Illumina HiSeq2000 platform. Depth of coverage, consistency of coverage across samples, and the overlap of variants identified were assessed. This data was also compared to whole-exome sequencing data from ten individuals.
Read depth was greater and less variable among samples that had been enriched using the RNA-bait hybridization capture enrichment method. In addition, samples enriched by hybridization capture had fewer exons with mean coverage less than 10, reducing the need for followup Sanger sequencing. Variants sets produced were 77% concordant, with both techniques yielding similar numbers of discordant variants.
When comparing the design flexibility, performance, and cost of the targeted enrichment methods to whole-exome sequencing, the RNA-bait hybridization capture enrichment gene panel offers the better solution for interrogating the aortopathy genes in a clinical laboratory setting.
KeywordsAortopathy Hybridization capture Marfan syndrome Next generation sequencing (NGS) Target enrichment Emulsion PCR
Aortopathies are a group of disorders characterized by aneurysms, dilation, and tortuosity of the aorta. Thoracic aortic aneurysm with dissection is the most common fatal condition involving the aorta , and can be syndromic, familial nonsyndromic, or sporadic. Over 20% of thoracic aneurysms result from inherited disorders . Syndromic connective tissue diseases with aortic involvment, such as Marfan syndrome (MFS; OMIM# 154700), Loeys-Dietz syndrome (LDS; OMIM# 609192), Ehlers Danlos syndrome type IV (EDS IV; OMIM# 130050), and congenital contractural arachnodactyly (OMIM# 121050) each result from mutations in different genes yet have broadly overlapping phenotypes [3–6]. In addition, mutations in genes related to the structure and function of the aortic wall, including MYH11[7, 8], ACTA2, SLC2A10, and NOTCH1, have been linked to non-syndromic familial forms of thoracic aortic aneurysms (reviewed in ).
Due to the overlapping phenotypes presented by these disorders genetic sequencing is often required for accurate diagnosis and appropriate clinical intervention. However, comprehensive sequencing of the many genes involved is often impractical with traditional Sanger methods. In contrast, high-throughput “next-generation” sequencing (NGS) has emerged as a new tool in the clinical laboratory for the rapid, cost-effective detection of mutations in genes associated with multigenic disorders [13, 14]. NGS assays targeted to a panel of genomic regions associated with known pathogenic mutations offer several advantages over traditional sequencing methods, including lower cost and rapid assessment of many regions.
While NGS offers a promising alternative to Sanger sequencing, the clinical utility of NGS methods depends on the ability to accurately isolate or amplify the genomic regions of interest. Ideally, the enrichment will yield high read depths in the targeted regions while keeping errors introduced through PCR and other sample manipulations to a minimum. Several NGS target enrichment strategies are currently available, all with various advantages depending on the size of the targeted region and the genetic targets themselves. Previous comparisons of enrichment techniques have identified considerable differences in the depth of coverage of targeted regions as well as the number of variants identified [15–17].
Aortopathy panel genes
No. reported mutations
Transforming growth factor, beta receptor 1
Loeys-Dietz syndrome; Marfan-like syndrome
Transforming growth factor, beta receptor 2
Loeys-Dietz syndrome; Marfan-like syndrome
Collagen type III alpha 1
Ehlers Danlos Type IV
Myosin heavy chain 11
TAAD b -patent ductus arteriosus
smooth muscle actin, alpha 2
solute carrier family 2 (facilitated glucose transporter), member 10
Arterial Tortuosity syndrome
Notch homolog 1, translocation-associated
NGS accuracy sample results
Coverage RD a (% mutant allele)
Coverage SS a (% mutant allele)
1477x (RD) 508x (SS)
In COL3A1, c.198A>G, p.I66M, a novel change.
462x (RD) 563x (SS)
177x (RD) 499x (SS)
140x (RD) 164x (SS)
No mutations found.
33x (RD) 260x (SS)
In SLC2A10 p.A206T (rs#2235491) and p.A385G (rs#79849424). In NOTCH1, IVS16-4C>CT (rs#3125001).
COL3A1 IVS9-7T>C novel change; potential splice site variant
86x (RD) 355x (SS)
In COL3A1, p.A698T (rs#1800255). In FBN2, p.V965I (rs#154001).
FBN1 Coriell positive
Unable to confirm due to sample quality.
Failed merge between DNA and primer library droplets
Poor data due to low quality DNA
No mutations found
Failed due pipetting error
This study was approved by the University of Utah and Primary Children’s Medical Center Institutional Review Boards (IRB#00028740). Written informed consent for participation in the study was obtained from the participants or their parents.
Emulsion PCR enrichment
A primer library (RainDance Technologies, Boston, MA) was custom-designed to amplify 194 exons and exon/intron boundaries for nine aortopathy genes listed in Table 1 (~0.1 Mb). Primers were designed using RainDance’s design parameters and Primer3 (http://primer3.sourceforge.net/). The 350 PCR amplicons ranged in size from 201–919 bp and had a guanine cytosine (GC) content of 24-80%. Genomic DNA (1.5-3 μg) was sheared to 2–4 kb fragments using a Covaris S2 instrument (Covaris, Woburn, MA) and added to a mixture that included all the components of the PCR reaction excluding the primers. This mixture and the primer library were loaded separately onto the RDT1000 instrument, and PCR droplets containing one primer pair per droplet were generated. After amplification, emulsion PCR droplets were broken releasing the amplicons which were then purified and concatenated according to the manufacturer’s instructions. Concatenated samples were sheared to 300 bp fragments, and Illumina adapters were added using the SPRI-TE instrument (Beckman-Coulter, Danvers, MA). Barcode indexes were added using PCR, and sample quality and quantity was assessed using a Bioanalyzer (Agilent Technologies, Santa Clara, CA).
Solution-based hybridization capture enrichment
Custom RNA baits were designed to specifically target the exons and exon/intron boundaries of the nine aortopathy genes from Table 1 (~0.1 Mb). RNA baits were tiled at 5 × spacing and were in replicates of 10 to increase hybridization efficiency. Genomic DNA (3 μg) was sheared to 180 bp fragments. Illumina adapters were added using SureSelect XT kit reagents (Agilent Technologies, Santa Clara, CA). Adapter ligated DNA underwent hybridization with the biotinylated RNA baits for 24 hours at 65°C. Hybridized DNA targets of interest were captured using streptavidin-coated magnetic beads. DNA targets of interest were eluted and barcode/indexed after a series of washes to remove the non-targeted, unbound genome. DNA quality and quantity was assessed (Bioanalyzer).
NGS sequencing and data analysis
Concentrations of indexed samples from the enrichments were determined by quantitative-PCR (KAPA Biosystems, Woburn, MA). Samples were pooled and sequenced on a HiSeq2000 instrument (Illumina, San Diego, CA) using 2 × 100 paired-end sequencing. Sequences were aligned to the human genome reference (hg19) sequence using the Burrows-Wheeler Alignment tool (BWA 0.5.9)  with default parameters. PCR duplicates were removed using the Samtools package , and base quality score recalibration, local realignment, and variant calling were performed with the Genome Analysis Toolkit (GaTK v1.3) . Variants with coverage less than 6, with 'quality-by-depth' scores of less than two, with variant allele frequencies of less than 0.15, or with overall quality scores of less than 10 were discarded.
Select variants were Sanger sequenced. Primers sequences are available upon request. Amplicons were bi-directly sequenced using the Big Dye® Terminator v3.1 cycle sequencing kit and an ABI 3730 DNA Analyzer (Life Technologies, Carlsbad, CA). Sequences were compared to reference sequences using Mutation Surveyor (SoftGenetics, State College, PA).
In addition to the custom targeted enrichments, we also examined depth of coverage for the targeted regions obtained through exome sequencing. Briefly, ten samples from individuals without known aortopathies were sequenced on the Illumina HiSeq2000 platform after undergoing exome capture enrichment (SureSelect 50Mb exome kit, Agilent Technologies, Santa Clara, CA). Exome samples were indexed and pooled, two per lane, using a one-to-one ratio, and were sequenced using 2x100-base pair paired end reads.
Six of eight samples underwent successful enrichment using both RainDance emulsion PCR and SureSelect hybridization capture (Table 2). Sample 7 (from Coriell) failed during the RDT1000 merge when the DNA droplets failed to merge properly with the primer library droplets. Hybridization capture of this sample was also poor and analysis was not completed due to sub-optimal DNA quality (A260/A280 was not between 1.8 and 2.0). Sample 8 failed during hybridization capture due to a pipetting error. Herein, the accuracy of the two enrichment methods, depth of coverage, and variant calling were compared among the six samples enriched using both techniques.
Pathogenic mutations from four positive control samples were detected using both enrichment methods (Table 2, samples 1–4). For example, a heterozygous FBN1 c.1585C > T, p.R529X mutation was detected in sample 1 using both enrichment methods and was confirmed using Sanger sequencing (Additional file 1: Figure S1).
Depth of coverage
Examining exons individuals, the SureSelect enrichment yielded fewer total exons with coverage below 10 when compared to both RainDance and the whole exome capture (Figure 1B). Some 98.5% of all exons had mean depth greater than 50 for SureSelect, as compared to 85.5% for RainDance and 70% for whole exome.
In addition to the number of reads mapping to targeted locations, we also assessed the quantity and character of the variants identified by both enrichment methods. Because both methods targeted overlapping, but partially distinct regions, we considered only those variants in the intersection of the two capture regions. The intersection of the two capture regions was 60.58 Kb in size, comprising 64% of the bases targeted by SureSelect and 99% of bases in the RainDance capture.
In total, 252 unique variants were identified across all samples, 195 of which were identified by both methods (Additional file 1: Table S1). The sequences generated by the RainDance enrichment contained several more variants, 221 in total, including 26 not identified in the SureSelect-enriched sequences. In contrast, the SureSelect protocol yielded 214 total variants, of which 19 were not found using the emulsion PCR technique. Among the variants common to both methods, 55 (28.2%) were in dbSNP (v. 132), while 2 of the 17 variants (12%) unique to SureSelect were in dbSNP, and 3 of the 23 variants (13%) unique to emulsion PCR were in dbSNP. Assuming variants found in dbSNP are not false positives in our samples, this suggests that even the relatively high coverage obtained by both enrichment methods did not guarantee accurate variant detection, as both the custom targeted enrichment methods identified several variants not recognized by the other method. Similarly discordant results have been found in other recent studies .
Examination of the discordant variants suggested several sources for the disagreement. First, discordant variants appeared in the intronic and untranslated areas at the periphery of individual capture regions. Ten SureSelect and fifteen RainDance discordant variants were located in non-exonic regions. Examination of read depth in these areas suggested that coverage was relatively low compared to the mean for the whole exon, implicating variation in read depth as a potential source of discordant or false positive calls.
A second source of discordant variation was the NOTCH1 gene. A relatively high fraction of the discordant variants appeared in the exons of NOTCH1 (15 of 45, or 33%), and these appeared with similar frequencies in both the RainDance and SureSelect samples (8 from RainDance, 7 from SureSelect). NOTCH1 is likely to be problematic for two reasons. First, the high GC content of NOTCH1 reduces the read depths obtained with the SureSelect enrichment procedure. Second, NOTCH1 bears substantial sequence homology to other NOTCH genes, potentially confounding alignment algorithms and generating spurious variant calls.
Clinical utility of the aortopathy panel
Two clinically affected individuals who had previously tested negative for a FBN1, TGFBR1, and TGFBR2 mutation by Sanger sequencing and MLPA  were evaluated using the aortopathy panel. Sample 5 was from a 19-year-old female with borderline aortic dilatation, mild tricuspid valve prolapse and mild mitral valve insufficiency, height of 178 cm (5’10”) (99th percentile), arm span of 176 cm (5’9”) severe pectus carinatum, scoliosis, arachnodactyly, joint mobility, positive wrist and thumb signs, and skin striae. Analysis of the coding region variants and splice site variants from both enrichment methods revealed two nonsynonymous heterozygous variants in SLC2A10 (c.616G > A, p.Ala206Thr and c.1154C > G, p. Ala385Gly) and a NOTCH1 potential splice site variant (IVS16-4C > T). These variants were present in dbSNP and are likely not disease causing.
Sample 6 was from a 34-year-old female with mild aortic dilatation in the setting of past aortic dissection and unknown family history. Two heterozygous nonsynonymous variants, one in COL3A1 (c.2092G > A, p.Ala698Thr) and the other in FBN2 (c.2893G > A, p.Val965Ile) were both present in dbSNP, rs1800255 and rs154001 at 19.8% and 72.4% frequencies in 1000 genomes (November 2010 release, 1000genomes.org), respectively. A novel heterozygous COL3A1 variant (IVS9-7T > C) not found in dbSNP or the COL3A1 locus specific mutation database was also identified. Splice site prediction programs were run to determine if the change altered splicing , but they predicted that no change in splicing would occur. It is likely that this variant is also not disease causing.
No synonymous splice site variants were identified in either patient. It is possible that these two patients have a large deletion or duplication of one of the nine aortopathy genes, but this has yet to be evaluated. Currently, detection of structural variation cannot be reliably detected from NGS data alone. Patient samples that test negative from the NGS assay should be run on an exonic level comparative genomic hybridization (CGH) array assay used to evaluate large copy number changes in the same gene set. Until NGS data analysis programs improve in their ability and accuracy to detect structural variation, CGH array assays are the suggested approach for detecting such large deletions or duplications in multi-gene panels. Alternatively, another gene not included in the aortopathy panel may be responsible for the disorder in these patients.
All targeted enrichment methods evaluated yielded very high read depths in the aortopathies targeted regions, with all genes except for NOTCH1 from the exome capture obtaining a mean depth of coverage of over 50 × (Figure 1). Overall, the custom-designed hybridization capture enrichment yielded lower coverage in regions of high GC content (Figure 2), but consistently high coverage in regions of low GC bias. The emulsion PCR method, in contrast, yielded exons with very low coverage that had no obvious correlate (Figure 2). Although influenced by GC content, the hybridization capture nonetheless yielded fewer total exons with low coverage than did the emulsion PCR technique (Figure 1B). Overall, emulsion PCR yielded significantly higher between-sample variability in coverage. In a clinical setting, a high degree of between sample consistency is desirable. Low coverage exons will likely require follow-up Sanger sequencing. Knowing which exons will require such treatment beforehand may ameliorate the cost and additional time resulting from designing new primer sets on an as-needed basis.
Despite satisfactory coverage, the methods yielded only partially overlapping sets of variants, with some 10% of variants identified by one method but not by the other (Additional file 1: Table S1). These inconsistencies may be related to variability in read depth as well as alignment ambiguity related to sequence homology. Similarly high discordancy rates were found in a recent study comparing the RainDance and Fluidigm enrichment platforms , in which only 42% of variants identified were common to both methods, and 67% of the discordant variants were found in regions of less than 20-fold coverage. Until procedures are devised that substantially decrease the number of regions with low coverage, Sanger confirmation of suspected variants will likely be necessary prior to clinical action.
A high sample failure rate of 25% (2 of 8 samples) was observed due to sample quality (sample 7, RainDance and SureSelect) and a pipetting error (sample 8, SureSelect) (Table 2). Based on our results, only DNA samples of high quality (A260/280 is between 1.8 and 2.0) should be evaluated using NGS enrichment. If a sample falls below this initial quality measurement, DNA should be extracted again and/or the sample should be purified until an acceptable quality is obtained. For complex enrichment protocols such as the SureSelect method, pipetting errors can be avoided by incorporating automation into sample preparation (discussed further below). This is especially important as the number of clinical samples increase.
NGS enrichment in the clinical laboratory
When compared to emulsion PCR, the solution-based hybridization technique yielded higher coverage as well as greater predictability in performance. These attributes, when combined with lower cost, strongly favor the hybridization capture for enrichment of the aortopathy panel in the clinical setting. Emulsion PCR enrichment is significantly more expensive and requires the use of a costly instrument. Exome sequencing is also more costly because the cost to sequence the exome at a high enough depth of coverage to accurately call variants is much higher than for the targeted panel. Samples can also be indexed and pooled prior to hybridization capture , reducing the overall cost per sample even further.
Despite the lower cost, the hybridization capture protocol is more time consuming, complex, and labor intensive when compared to the emulsion PCR technique. However, the ability to automate this process in the clinical laboratory alleviates these challenges and yields more consistent, reliable results . Currently, the emulsion PCR instrument processes one sample/hour, limiting the total number of samples processed in a day to approximately twenty-four. With the increasing interest in exome sequencing, automation of hybridization capture enrichment protocols have become readily available , and up to 96 different custom-designed NGS panels and exome hybridizations can take place at the same time during the same run. Solution-based hybridization libraries can be ordered in larger volumes, aliquoted for up to two-three freeze/thaw cycles, and stored for up to one year. RainDance primer libraries have to be switched out after the eighth sample with the RDT1000 instrument design, and if there are not eight samples to run using the same primer library, the rest will be wasted.
The aortopathy panel offers a cost-effective, faster molecular diagnostic assay compared to the conventional gene-by-gene Sanger sequencing approach. Both RainDance and SureSelect enrichment methods accurately identified variants in the positive samples assayed, and overall coverage was high across most exons for all samples. Hybrid capture results demonstrated that genes with high homology to other genes and high GC rich regions can be problematic. However, we note that increases in read length, currently available with the Ion Torrent or Illumina MiSeq instruments, are likely to alleviate homology-related alignment issues. After comparing the cost, design flexibility, and versatility of the workflows of the two enrichment methods, the custom-designed hybridization capture design offers the best solution for enriching the aortopathy genes in a clinical laboratory setting. Patients with Marfan syndrome and Marfan-like syndromes featuring aortopathies will benefit from the timely molecular diagnosis of this new testing approach which will lead to the appropriate surveillance and interventions aimed at preventing the significant morbidity and mortality associated with these conditions.
We thank Lin Pham, Ph.D. for her assistance in the design of the RainDance library. We thank Owen Hardy, Maria Celeste-Ramirez, and Christopher Hopkins, Ph.D. for providing assistance in the design of the SureSelect library. This work was funded by the ARUP Institute for Clinical and Experimental Pathology.
- Klein DG: Thoracic aortic aneurysms. J Cardiovasc Nurs. 2005, 4: 245-250.View ArticleGoogle Scholar
- Verloes A, Sakalihasan N, Koulischer L, Limet R: Aneurysms of the abdominal aorta: familial and genetic aspects in three hundred thirteen pedigrees. J Vasc Surg. 1995, 21: 646-655. 10.1016/S0741-5214(95)70196-6.View ArticlePubMedGoogle Scholar
- Law C, Bunyan D, Castle B, Day L, Simpson I, Westwood G, Keeton B: Heterozygous TGFΒR2 mutations in Marfan syndrome. Nat Genet. 2004, 36: 855-860. 10.1038/ng1392.View ArticleGoogle Scholar
- Loeys BL, Chen J, Neptune ER, Judge DP, Podowski M, Holm T, Meyers J, Leitch CC, Katsanis N, Sharifi N, Xu FL, Myers LA, Spevak PJ, Cameron DE, De Backer J, Hellemans J, Chen Y, Davis EC, Webb CL, Kress W, Coucke P, Rifkin DB, De Paepe AM, Dietz HC: A syndrome of altered cardiovascular, craniofacial, neurocognitive and skeletal development caused by mutations in TGFΒR1 or TGFΒR2. Nat Genet. 2005, 37: 275-281. 10.1038/ng1511.View ArticlePubMedGoogle Scholar
- Pannu H, Fadulu VT, Chang J, Lafont A, Hasham SN, Sparks E, Giampietro PF, Zaleski C, Estrera AL, Safi HJ, Shete S, Willing MC, Raman CS, Milewicz DM: Mutations in transforming growth factor-β receptor type II cause familial thoracic aortic aneurysms and dissections. Circulation. 2005, 112: 513-520. 10.1161/CIRCULATIONAHA.105.537340.View ArticlePubMedGoogle Scholar
- Gupta PA, Wallis DD, Chin TO, Northrup H, Tran-Fadulu VT, Towbin JA, Milewicz DM: FBN2 mutation associated with manifestations of Marfan syndrome and congenital contractural arachnodactyly. J Med Genet. 2004, 41: e56-10.1136/jmg.2003.012880.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhu L, Vranckx R, Khau Van Kien P, Lalande A, Boisset N, Mathieu F, Wegman M, Glancy L, Gasc JM, Brunotte F, Bruneval P, Wolf JE, Michel JB, Jeunemaitre X: Mutations in myosin heavy chain 11 cause a syndrome associating thoracic aortic aneurysm/aortic dissection and patent ductus arteriosus. Nat Genet. 2006, 38: 343-39. 10.1038/ng1721.View ArticlePubMedGoogle Scholar
- Khau Van Kien P, Mathieu F, Zhu L, Lalande A, Betard C, Lathrop M, Brunotte F, Wolf JE, Jeunemaitre X: Mapping of familial thoracic aortic aneurysm/dissection with patent ductus arteriosus to 16p12.2–p13.13. Circulation. 2005, 112: 200-206. 10.1161/CIRCULATIONAHA.104.506345.View ArticlePubMedGoogle Scholar
- Guo DC, Pannu H, Tran-Fadulu V, Papke CL, Yu RK, Avidan N, Bourgeois S, Estrera AL, Safi HJ, Sparks E, Amor D, Ades L, McConnell V, Willoughby CE, Abuelo D, Willing M, Lewis RA, Kim DH, Scherer S, Tung PP, Ahn C, Buja LM, Raman CS, Shete SS, Milewicz DM: Mutations in smooth muscle α-actin (ACTA2) lead to thoracic aortic aneurysms and dissections. Nat Genet. 2007, 39: 1488-1493. 10.1038/ng.2007.6.View ArticlePubMedGoogle Scholar
- Coucke PJ, Willaert A, Wessels MW, Callewaert B, Zoppi N, De Backer J, Fox JE, Mancini GM, Kambouris M, Gardella R, Facchetti F, Willems PJ, Forsyth R, Dietz HC, Barlati S, Colombi M, Loeys B, De Paepe A: Mutations in the facilitative glucose transporter GLUT10 alter angiogenesis and cause arterial tortuosity syndrome. Nat Genet. 2006, 38: 452-457. 10.1038/ng1764.View ArticlePubMedGoogle Scholar
- Garg V, Muth AN, Ransom JF, Schluterman MK, Barnes R, King IN, Grossfeld PD, Srivastava D: Mutations in NOTCH1 cause aortic valve disease. Nature. 2005, 437: 270-274. 10.1038/nature03940.View ArticlePubMedGoogle Scholar
- El-Hamamsy I, Yacoub MH: Cellular and molecular mechanisms of thoracic aortic aneurysms. Nat Rev Cardiol. 2009, 6: 771-786. 10.1038/nrcardio.2009.191.View ArticlePubMedGoogle Scholar
- Jones MA, Bhide S, Chin E, Ng BG, Rhodenizer D, Zhang VW, Sun JJ, Tanner A, Freeze HH, Hegde MR: Targeted polymerase chain reaction-based enrichment and next generation sequencing for diagnostic testing of congenital disorders of glycosylation. Genet Med. 2011, 13: 921-932. 10.1097/GIM.0b013e318226fbf2.View ArticlePubMedPubMed CentralGoogle Scholar
- Berg JS, Evans JP, Leigh MW, Omran H, Bizon C, Mane K, Knowles MR, Weck KE, Zariwala MA: Next generation massively parallel sequencing of targeted exomes to identify genetic mutations in primary ciliary dyskinesia: implications for application to clinical testing. Genet Med. 2011, 13: 218-229. 10.1097/GIM.0b013e318203cff2.View ArticlePubMedPubMed CentralGoogle Scholar
- Teer JK, Bonnycastle LL, Chines PS, Hansen NF, Aoyama N, Swift AJ, Abaan HO, Albert TJ, Comparative Sequencing Program NISC, Margulies EH, Green ED, Collins FS, Mullikin JC, Biesecker LG: Systematic comparison of three genomic enrichment methods for massively parallel DNA sequencing. Genome Res. 2010, 20: 1420-1431. 10.1101/gr.106716.110.View ArticlePubMedPubMed CentralGoogle Scholar
- Kiialainen A, Karlberg O, Ahlford A, Sigurdsson S, Lindblad-Toh K, Syvänen AC: Performance of microarray and liquid based capture methods for target enrichment for massively parallel sequencing and SNP discovery. PLoS One. 2011, 6: e16486-10.1371/journal.pone.0016486.View ArticlePubMedPubMed CentralGoogle Scholar
- Hedges DJ, Guettouche T, Yang S, Bademci G, Diaz A, Andersen A, Hulme WF, Linker S, Mehta A, Edwards YJ, Beecham GW, Martin ER, Pericak-Vance MA, Zuchner S, Vance JM, Gilbert JR: Comparison of three targeted enrichment strategies on the SOLiD sequencing platform. PLoS One. 2011, 6: e18595-10.1371/journal.pone.0018595.View ArticlePubMedPubMed CentralGoogle Scholar
- Tewhey R, Warner JB, Nakano M, Libby B, Medkova M, David PH, Kotsopoulos SK, Samuels ML, Hutchison JB, Larson JW, Topol EJ, Weiner MP, Harismendy O, Olson J, Link DR, Frazer KA: Microdroplet-based PCR enrichment for large-scale targeted sequencing. Nat Biotechnol. 2009, 27: 1025-1031. 10.1038/nbt.1583.View ArticlePubMedPubMed CentralGoogle Scholar
- Gnirke A, Melnikov A, Maguire J, Rogov P, LeProust EM, Brockman W, Fennell T, Giannoukos G, Fisher S, Russ C, Gabriel S, Jaffe DB, Lander ES, Nusbaum C: Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing. Nat Biotechnol. 2009, 27: 182-189. 10.1038/nbt.1523.View ArticlePubMedPubMed CentralGoogle Scholar
- Furtado LV, Wooderchak-Donahue W, Rope AF, Yetman AT, Lewis T, Plant P, Bayrak-Toydemir P: Characterization of large genomic deletions in the FBN1 gene using multiplex ligation-dependent probe amplification. BMC Med Genet. 2011, 12: 119-10.1186/1471-2350-12-119.View ArticlePubMedPubMed CentralGoogle Scholar
- Li H, Durbin R: Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics. 2009, 25: 1754-1760. 10.1093/bioinformatics/btp324.View ArticlePubMedPubMed CentralGoogle Scholar
- Li H, Ruan J, Durbin R: Mapping short DNA sequencing reads and calling variants using mapping quality scores. Genome Res. 2008, 18: 1851-1858. 10.1101/gr.078212.108.View ArticlePubMedPubMed CentralGoogle Scholar
- McKenna A, Hanna M, Banks E, Sivachenko A, Cibulskis K, Kernytsky A, Garimella K, Altshuler D, Gabriel S, Daly M, DePristo MA: The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data. Genome Res. 2010, 20: 1297-1303. 10.1101/gr.107524.110.View ArticlePubMedPubMed CentralGoogle Scholar
- Cartegni L, Wang J, Zhu Z, Zhang MQ, Krainer AR: ESEfinder: a web resource to identify exonic splicing enhancers. Nucelic Acids Res. 2003, 31: 3568-3571. 10.1093/nar/gkg616.View ArticleGoogle Scholar
- Bell CJ, Dinwiddie DL, Miller NA, Hateley SL, Ganusova EE, Mudge J, Langley RJ, Zhang L, Lee CC, Schilkey FD, Sheth V, Woodward JE, Peckham HE, Schroth GP, Kim RW, Kingsmore SF: Carrier testing for severe childhood recessive diseases by next generation sequencing. Sci Transl Med. 2011, 3: 65ra4-10.1126/scitranslmed.3001756.View ArticlePubMedPubMed CentralGoogle Scholar
- Fisher S, Barry A, Abreu J, Minie B, Nolan J, Delorey TM, Young G, Fennell TJ, Allen A, Ambrogio L, Berlin AM, Blumenstiel B, Cibulskis K, Friedrich D, Johnson R, Juhn F, Reilly B, Shammas R, Stalker J, Sykes SM, Thompson J, Walsh J, Zimmer A, Zwirko Z, Gabriel S, Nicol R, Nusbaum C: A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries. Genome Biol. 2011, 12: R1-10.1186/gb-2011-12-1-r1.View ArticlePubMedPubMed CentralGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1755-8794/5/50/prepub
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