Figure 2

Network analysis and comparison of gene expression patterns. Ingenuity Pathways analysis (IPA) was used to generate the network, which has been overlaid with relative gene expression levels of directly irradiated cells (a) and their non-irradiated bystanders (b). Nodes representing gene products are displayed by cellular localization (extracellular space, plasma membrane, cytoplasm or nucleus). The intensity of each node's color indicates the expression level (scale bar) relative to untreated controls. Up-regulated genes are red and down-regulated genes green. Edges (lines and arrows between nodes) represent direct interactions between molecules as supported by information in the Ingenuity knowledge base. Light blue edges highlight direct interactions with p53 (panel a) and NFkB (panel b). The shape of a node represents the functional class of the gene product; rectangles with solid lines for cytokines, rectangles with dotted lines for growth factors, triangles for phosphatases, concentric circles for groups or complexes, diamonds for enzymes, and ovals for transcriptional regulators or modulators.