Genomic selection of reference genes for real-time PCR in human myocardium

BMC Medical Genomics

Table 1 Real-time PCR primers

Primer		Sequence	Tm	Length (bp)	Efficiency (%)
GAPDH	Forward	GCTCATTTCCTGGTATGACAACG	63	213	92.8
	Reverse	AGGGGTCTACATGGCAACTG	60
RPL22	Forward	CCATGGCTCCTGTGAAAAAG	61	219	91.6
	Reverse	TCACGGTGATCTTGCTCTTG	60
TPT1	Forward	AAATGTTAACAAATGTGGCAATTAT	58	164	95.9
	Reverse	AACAATGCCTCCACTCCAAA	61
RPS4X	Forward	GATCCCCTCATCAAGGTGAA	60	243	78.7
	Reverse	GCCCTTGCCAATAACAAAAA	60
RPL13A	Forward	CGCCCTACGACAAGAAAAAG	60	206	96.9
	Reverse	CCGTAGCCTCATGAGCTGTT	60
RPL23A	Forward	GCTCCCAGGAGAAACAAGC	60	201	92.2
	Reverse	ATCAGGCCGAATCAGGGTGTT	65
EEF1A1	Forward	CTTTGGGTCGCTTTGCTGTT	63	183	95.0
	Reverse	CCGTTCTTCCACCACTGATT	60
RPL41	Forward	ATGAGAGCCAAGTGGAGGAA	60	219	94.0
	Reverse	TCAGAGGGCGATGAAGTTCT	60
RNPS1	Forward	ACCCATGGTAGTTGCTGCTC	60	104	95.3
	Reverse	AGCTGGCTCTCCACTCACTC	60
SRP14	Forward	CAGATGGCTTATTCAAACCTCCT	61	181	99.9
	Reverse	ATGCCCTTTACTGTGCTGCT	60

ISSN: 1755-8794