Figure 2

Histograms depict expression levels of AQP8, HPGD, PRDX6, MXI1, NDRG2, SCNNB1 and SGK2 genes in CaCo2 cell line (A), HCT116 cell line (B) and SW480 cell line (C) before and after exposure to 5-Aza-CdR determined by quantitative real-time PCR using the Comparative CT method. 2^{^DCt} indicates the ratio between the values of CT normalized to three housekeeping genes and compared to cDNA of untreated cells used as calibrator. Error bars indicate standard deviation from triplicate experiments. p16 gene was used as positive control in the experiments. Statistical significance threshold p-value (p < 0.001) for both acute and chronic treatment are shown. Asterisks indicate P < 0.05 values and double asterisks indicate P < 0.001 values.