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Table 3 Luminex-based multiplex cytokine assay showing the expression of chemokines and cytokines in the supernatants of HUVEC Vector and K13-ER cells with and without 4OHT treatment and following infection with KSHV.

From: Integrated microarray and multiplex cytokine analyses of Kaposi's Sarcoma Associated Herpesvirus viral FLICE Inhibitory Protein K13 affected genes and cytokines in human blood vascular endothelial cells

Cytokines Vector Mock treated Vector + 4OHT K13-ER-Mock treated K13-ER+4OHT KSHV Virus Fold change K13-ER Fold change KSHV Fold change based on Microarray data
IL-1b 155.5 193.5 148 200 200 1.3 1.3 1.07
IL-2 20 23 20 22 21 1.1 1.05 1.01
IL-4 15 15 16 23 17 1.4 1.06 -2.9
IL-5 5 5 4 7 5 1.75 1.25 1.2
IL-6 1946.5 2038 1043.5 10910 11242 10.45 10.77 21.17
IL-8 6115 6682 4459.5 10336 10094.5 2.31 2.26 31.5
IL-10 1023 985 1003 1011.5 1038.5 1.00 1.03 3.3
IFN-γ 24.5 20 21 31.5 27 1.5 1.28 1.9
CSF2 13 15 10 1938 51 193.80 5.10 15.1
TNF-α 8 10 9 24 14 2.66 1.55 5.6
EOTAXIN 5 6 4 10 6 2.50 1.50 3.4
MCP-1 3217 2928 85 13178 10235.5 155.03 120.41 71.4
MIP-1α 37.5 31 24 117 105 4.87 4.37 3.6
MIP-1β 38 43 42.5 65 58 1.52 1.36 1.4
CCL5 327 345.5 305.5 11126.5 197 36.42 0.644 48.3
EGF 8798 8695 8711 8962 8765 1.02 1.00 16.5
VEGF 421 491 466.5 479 447 1.02 0.95 2.3
FGF-β 415 428 515 615.5 500 1.19 0.97 10.7
G-CSF 95 104 36 2125 604 59.02 16.77 3.9
HGF 134.5 143.5 148.5 155.5 264 1.04 1.77 3.2
CXCL10 498.5 485.5 70 10453 2259 149.32 32.27 47.7
IL-12p40 21 18.5 16.5 197 34 11.93 2.06 97
IL-13 7 8 7 13 8 1.85 1.14 1.4
IL-15 11 12 10 39 12 3.90 1.20 7.8
IL-17 118 139 130 129 132 0.99 1.01 -1.3
IFN-α 56 60 40 131 98 3.27 2.45 1.4
MIG 13 12 14 39 37.5 2.78 2.67 3.8
IL-1α 454.5 521.5 469 752 494.5 1.60 1.05 2.3
IL-7 58 50 47 197 181.5 4.19 3.86 2.1
IL-1Rα 30 31 28.5 90 59.5 3.15 2.08 3.8
IL-2R 12 16 15 21.5 18 1.43 1.20 2.6
GROα 13 13 14 110 17 7.85 1.21 7.7
MCP-2 26.5 25 23 37 23 1.60 1.00 4.4
MCP-3 12 14 10 23 372 2.30 37.20 2.3