Integrated microarray and multiplex cytokine analyses of Kaposi's Sarcoma Associated Herpesvirus viral FLICE Inhibitory Protein K13 affected genes and cytokines in human blood vascular endothelial cells

Table 3 Luminex-based multiplex cytokine assay showing the expression of chemokines and cytokines in the supernatants of HUVEC Vector and K13-ER cells with and without 4OHT treatment and following infection with KSHV.

Cytokines	Vector Mock treated	Vector + 4OHT	K13-ER-Mock treated	K13-ER+4OHT	KSHV Virus	Fold change K13-ER	Fold change KSHV	Fold change based on Microarray data
IL-1b	155.5	193.5	148	200	200	1.3	1.3	1.07
IL-2	20	23	20	22	21	1.1	1.05	1.01
IL-4	15	15	16	23	17	1.4	1.06	-2.9
IL-5	5	5	4	7	5	1.75	1.25	1.2
IL-6	1946.5	2038	1043.5	10910	11242	10.45	10.77	21.17
IL-8	6115	6682	4459.5	10336	10094.5	2.31	2.26	31.5
IL-10	1023	985	1003	1011.5	1038.5	1.00	1.03	3.3
IFN-γ	24.5	20	21	31.5	27	1.5	1.28	1.9
CSF2	13	15	10	1938	51	193.80	5.10	15.1
TNF-α	8	10	9	24	14	2.66	1.55	5.6
EOTAXIN	5	6	4	10	6	2.50	1.50	3.4
MCP-1	3217	2928	85	13178	10235.5	155.03	120.41	71.4
MIP-1α	37.5	31	24	117	105	4.87	4.37	3.6
MIP-1β	38	43	42.5	65	58	1.52	1.36	1.4
CCL5	327	345.5	305.5	11126.5	197	36.42	0.644	48.3
EGF	8798	8695	8711	8962	8765	1.02	1.00	16.5
VEGF	421	491	466.5	479	447	1.02	0.95	2.3
FGF-β	415	428	515	615.5	500	1.19	0.97	10.7
G-CSF	95	104	36	2125	604	59.02	16.77	3.9
HGF	134.5	143.5	148.5	155.5	264	1.04	1.77	3.2
CXCL10	498.5	485.5	70	10453	2259	149.32	32.27	47.7
IL-12p40	21	18.5	16.5	197	34	11.93	2.06	97
IL-13	7	8	7	13	8	1.85	1.14	1.4
IL-15	11	12	10	39	12	3.90	1.20	7.8
IL-17	118	139	130	129	132	0.99	1.01	-1.3
IFN-α	56	60	40	131	98	3.27	2.45	1.4
MIG	13	12	14	39	37.5	2.78	2.67	3.8
IL-1α	454.5	521.5	469	752	494.5	1.60	1.05	2.3
IL-7	58	50	47	197	181.5	4.19	3.86	2.1
IL-1Rα	30	31	28.5	90	59.5	3.15	2.08	3.8
IL-2R	12	16	15	21.5	18	1.43	1.20	2.6
GROα	13	13	14	110	17	7.85	1.21	7.7
MCP-2	26.5	25	23	37	23	1.60	1.00	4.4
MCP-3	12	14	10	23	372	2.30	37.20	2.3

ISSN: 1755-8794