Verification of genes differentially expressed in neuroblastoma tumours: a study of potential tumour suppressor genes

Table 3 QPCR results of genes selected for the second verifiaction round

Gene	Selection criteria	FC Ver 1	t-test Ver 1	FC Ver 2	t-test Ver 2
ATBF1	p-value	9	1.70E-05	2.7	0.032
CACNA2D3	FC	133	0.0019	5.1	0.049
CNTNAP2	FC	287	0.0011	5.1	0.0036
DCUN1D2	FC	26	0.0027	1.0	0.46
DPYSL3	FC	23	0.01	1.6	0.19
FUSIP1	LOH	22	0.013	2.0	0.013
GNB1	PR	10	0.0028	2.3	0.007
POU4F2	PR; FC	1518	0.011	3.7	0.15
RAPGEF6	PR	37	0.0097	1.2	0.36
SLC35E2	PR	3	0.024	3.5	0.011
SLC6A2	FC	540	0.0012	2.8	0.11
TFAP2B	PR	13	0.012	4.5	0.0049

Column 2: p-value = Most significant in Ver 1 and cancer involvement, FC = Top ten with highest fold change (FC), LOH = Localized in a common Loss of Heterozygosity (LOH) region, PR = Previously Reported in NB microarray expression studies; column 3–4: Ver 1 = verification group 1; column 5–6: Ver 2 = verification group 2;

ISSN: 1755-8794