Examining smoking-induced differential gene expression changes in buccal mucosa

BMC Medical Genomics

Table 3 qPCR results comparing blood and buccal RNA across four genes with WT amplified template

	TMEM8			ANKRD			ITGA5		RPS3A
	_2067*	_1372	_584	_4387	_2552	_1345	_3879	_1994	_198
Subject
Buccal 1	33.76	No Ct	34.93	No Ct	30.33	28.91	31.7	No Ct	31.86
Buccal 2	29.72	29.15	26.83	31.64	29.52	27.47	No Ct	No Ct	28.65
Buccal 3	28.18	30.52	29.27	30.81	35.73	30.13	No Ct	38.64	28.49
Buccal 4	24.96	27.74	28.09	31.8	33.55	31.98	35.71	No Ct	26.82
Buccal 5	25.31	26.56	26.39	31.36	32.8	34.02	No Ct	No Ct	27.38
Buccal 6	35.22	34.85	34.1	No Ct	No Ct	37.42	No Ct	No Ct	ND
Buccal 7	29.05	29.24	35.31	No Ct	No Ct	No Ct	No Ct	No Ct	33.19
Mean	29.46	29.68	30.7	31.4	32.39	31.66	33.71	38.64	394
StDev	3.61	2.63	3.65	0.38	2.24	3.32	2.01	0	2.33
Blood 1	27.03	26.49	25.78	30.58	29.28	30.36	24.86	26.67	26.31
Blood 2	28.5	28.28	26.58	29.04	28.87	28.86	24.41	26.42	26.66
Blood 3	28.08	27.71	26.7	29.03	29.93	28.33	24.28	25.34	26.1
Blood 4	27.64	26.53	26.57	29.43	29.61	29.68	25.14	25.62	27.11
Blood 5	27.3	27.63	25.32	29.82	28.71	28.89	24.72	25.29	26
Blood 6	27.96	27.67	26.02	29.68	29.09	29.45	26.69	28.92	ND
Blood 7	27.8	28.48	27.62	29.49	28.73	28.06	24.34	27.14	26.82
Mean	27.76	27.57	26.37	29.57	29.17	29.09	24.92	26.49	26.5
StDev	0.46	0.78	0.69	0.48	0.43	0.74	0.78	1.19	0.4
Controls^
NTC	39.4	No Ct	No Ct	No Ct	No Ct	No Ct	No Ct	No Ct	No Ct
WT amplification	28.4	27.28	26.45	29.47	29.23	29.48	24.63	26.59	2594
3' amplification	26			22.9			24.4		20.5
Product (bp)	179	110	118	199	150	143	104	107	276
mRNA (bp)	2529	2529	2529	6339	6339	6339	4248	4248	903

Results are in Ct values from the four genes used in initial expression assessment by qPCR. Results shown are the average value from three replicates for each primer pair as input material from each subject.
* Primers used in qPCR are given by gene heading followed by 5' position of amplification on mRNA. See Additional file 1.
^ NTC, No template control; WT whole transcriptome, and 3' Positive Controls are RNA from pooled blood samples
(ND, not done; bp, basepair)

ISSN: 1755-8794