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Table 2 Real Time PCR Analysis of Selected Genes in Subcutaneous and Omental LAT and HAT Groups

From: Association of adipocyte genes with ASP expression: a microarray analysis of subcutaneous and omental adipose tissue in morbidly obese subjects

Gene Accession # Subcutaneous Omental
   LAT HAT % LAT HAT %
C5L2 NM_018485 0.950 ± 0.105 1.958 ± 0.237** 206% (NA) 1.161 ± 0.096 2.684 ± 0.263** 231% (NA)
C3 NM_000064 0.588 ± 0.086 0.928 ± 0.117* 158% (134%) 2.655 ± 0.829 2.344 ± 0.026 88% (45%)
C5aR NM_00173 1.412 ± 0.189 1.588 ± 0.120 112% (78%) 1.738 ± 0.240 1.926 ± 0.229 111% (122%)
CD36 NM_000072 0.877 ± 0.134 1.717 ± 0.212** 196% (268%) 0.411 ± 0.072 0.874 ± 0.103** 213% (96%)
DGAT2 NM_032564 1.671 ± 0.358 3.383 ± 0.513* 202% (245%) 0.606 ± 0.207 1.110 ± 0.151* 183% (144%)
FABP4 NM_001442 0.732 ± 0.093 0.776 ± 0.075 106% (128%) 0.638 ± 0.064 0.739 ± 0.083 116% (92%)
CEBPγ NM_001806 1.006 ± 0.226 1.497 ± 0.126* 160% (143%) 3.371 ± 1.694 3.607 ± 1.318 97% (75%)
INSR X02160 1.553 ± 0.278 1.580 ± 0.252 102% (112%) 1.407 ± 0.617 1.630 ± 0.371 116% (62%)
  1. Gene expression in SC and OM adipose tissue from Lower ASP and TG (LAT) and Higher ASP and TG (HAT) subjects as assessed by ΔΔ real time RT-PCR relative to the housekeeping gene B2M. Data are means ± SEM (n = 8 to 16 total per group) and are analysed by t test, where * p < 0.05, and ** p < 0.01. Percentage change of HAT vs LAT is provided, with the comparative value from microarray analysis (see Figures and Additional File 1, Table S1 for data) given in parentheses, where NA = not available.