Multiplex PCR for intactness of AZFb region and SNV analysis in the amplicons and genes. a-c shows representative gel pictures of multiples PCR used for checking the intactness of AZFb region. The sample IDs are shown on top while the amplicon size along with the corresponding STSs is given on right. None of the samples showed any deletion in this region for the STSs screened. d-g are the results of representative samples for g, g1, g2 and g3 SFVs respectively. h-i represents SNV results for selective samples for TTY4 and BPY2, respectively. For fragment length, restriction enzymes and other details refer to table 2.