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Figure 1 | BMC Medical Genomics

Figure 1

From: Concomitant heterochromatinisation and down-regulation of gene expression unveils epigenetic silencing of RELBin an aggressive subset of chronic lymphocytic leukemia in males

Figure 1

Epigenetic modifications of histone H3 and RelB expression: histone H3K9 trimethylation in the RELB promoter (using ChIP and Q-PCR). Chromatin was immunopurified by anti-3meH3K9 immunoprecipitation (ChIP) and the levels of 3metH3K9 in the three promoter regions of RELB were established by Q-PCR SYBR Green. These levels were expressed as the percentage of the input chromatin fraction defined in Material and Method section. Ns: non significant. The sequences of the primers used for amplifying the three indicated RELB promoter regions are: 74 bp amplicon: forward primer 5' CTGGTGATAGGGATGAGC 3' and reverse primer 5' CGAATGGCAGCAGTGTA 3'; 121 bp amplicon: forward primer 5' GGGTTACAACAACGCACAA 3' and reverse primer 5' CCTCCAAGGTCTCGCTAC 3'; 58 bp amplicon: forward primer 5' TGCTCAATGGGTAAGGC 3' and reverse primer 5' TGCTCTGGACGAGACAAC3'.

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BMC Medical Genomics

ISSN: 1755-8794

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