NGS data analysis pipeline and comparison of sRNA annotations in MDS. NGS data analysis pipeline used for this study. In A) we show the annotation of a sequence read. It was detected about 18000 times in RAEB2 and aligned at nine different positions, spread over six chromosomes, on the human genome (green). A single alignment position is shown (red) with the used annotation hierarchy (blue). The purple callbox, details the matched loci for miRNA let-7a-1, its full primary sequence (top), its mature sequence (middle) and the aligned short read (bottom). The brown callbox shows all nine annotations, including a number of miRNAs from the has-let-7 family as well as a piRNA. In B) we compare the total RNA content measured from our high-throughput sequencing and annotation steps, on the left results for the RAEB2, in the middle results for RA and on the right results for control.