Figure 3From: Exon array analysis reveals neuroblastoma tumors have distinct alternative splicing patterns according to stage and MYCN amplification status Alternative splicing of pyruvate kinase (PKM2) detected by Affymetrix exon (HuEx) array. (A) Gene structure of known isoforms is shown on the top panel with predicted domains/motifs that differ in protein isoforms. Green oval shows intersubunit contact (ISC) sequence, and red boxes point to fructose 1,6-bisphosphate (FBP) binding regions as defined by UniProt. The HuEx expression is shown on the bottom panel. Each point represents mean log2-expression of each group that was then median-centered across three groups. Orange lines point to probeset 3631984 that mapped to the unique exon in isoform M1 (NM_182470 and NM_182471), and probeset 3631977 that mapped to the unique exon in isoform M2 (NM_002654). While isoform M2-specific probeset showed increased expression in stage 4+ compared to stage 4-/1- tumors, isoform M1-specific probeset indicated lower expression in stage 4+ tumor. (B) Normalized intensity (NI) values for probeset 3631984 in Stage 1-, 4- and 4+ tumors. (C) Normalized intensity values for probeset 3631977 in Stage 1-, 4- and 4+ tumors. The expression for probesets 3631984 and 3631977was significantly different between stage 1- and 4+, suggesting the increased expression of isoform M2 and reduced expression of isoform M1 in MYCN-amplified neuroblastoma.Back to article page