Mixing experiment to assess efficiency of HRM for detection of differential allelic expression. (A) SimpleProbe® melting curves generated on the LightScanner® instrument from mixing series of opposite homozygous genomic DNAs for the marker SNP rs2236142 in CHEK2. Mixing ratios are indicated on the figure (G allele: C allele ratio). (B) The determination coefficient (R2) between the expected and the observed allelic ratios was 0.963. Each value corresponds to the mean value of 4 replicate measurements.