Examples of myogenesis-specific immunostaining of FSHD and control myoblasts and myotubes used for the analysis. Green, desmin; red, myosin heavy chain (MF20); blue, DAPI. The last panels in both rows were myotube preparations doubly stained with MF20 and DAPI. The occasional small bright green or red dots are a staining artifact. Fibroblasts do not stain with the desmin antibody nor with MF20.