Table 2 Primer sequences, position, product length, and CpG units used for MassArray quantitative methylation analysis
From: LINE-1 methylation status and its association with tetralogy of fallot in infants
Genes | Forward primer (5′ → 3′)1 | Reverse primer (5′ → 3′)2 | Position | Product length (bp) | CpG unit |
|---|---|---|---|---|---|
LINE-1 | TTTTATTAGGGAGTGTTAGATAGTGGG | CCCCAAAAATAAAACCTACAAAAAC | −835–-368 | 468 | 24 |
NKX2-5 | AGGAGGGTTTTGGATTTTTTTT | ATTTATTCCCAAACCTCTACTCCTC | −59–426 | 486 | 21 |
HAND1 | GAGGAGATTTGTTGGTTAGATGTTT | AATAAAAATTCCAACAATTCCCAAT | −886–-414 | 473 | 25 |
TBX20 | TTTGAGTGTGTATGTTAGTTTGAGTTT | CTCCTATTTTCCCTAAAAAAACCCT | −945–-635 | 311 | 17 |