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Figure 4 | BMC Medical Genomics

Figure 4

From: Screening significantly hypermethylated genes in fetal tissues compared with maternal blood using a methylated-CpG island recovery assay-based microarray

Figure 4

Methylations of PITX2, TLX3 , OTX2 and MNX1 genes validated using the combined bisulfite restriction analysis. Bisulfite converted genomic DNA was amplified with gene-specific primers followed by digestion with a methylation sensitive enzyme BstUI (CG↓CG). Methylation status was measured in placental tissue (P), maternal peripheral blood (M) and chorionic villus (C). The expected enzyme-digested pattern of the PCR products in which CpG islands were fully methylated was depicted on right side.

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