Skeletal muscle alterations and exercise performance decrease in erythropoietin-deficient mice: a comparative study

Mille-Hamard, Laurence; Billat, Veronique L; Henry, Elodie; Bonnamy, Blandine; Joly, Florence; Benech, Philippe; Barrey, Eric

doi:10.1186/1755-8794-5-29

Table 1 List of the primers used for RT-qPCR

From: Skeletal muscle alterations and exercise performance decrease in erythropoietin-deficient mice: a comparative study

Genes	Primers	Sequences 5'- > 3'	Length	Tm	%GC
Mcptl	Fwd	TTCTGGACGCGGAAATGC	18	59	56
	Rev	CTGTTAATCCAGGGCACATGTG	22	59	50
Runx1	Fwd	GCCATTTTGGGTCTGCTTTC	20	58	50
	Rev	TTGTCTGCGCAGGACGTTT	19	59	53
Plcb2	Fwd	ACCGCATTGATGTGGTGGTA	20	58	50
	Rev	TGCGTACACTGCGCTCTGA	19	59	58
Ccdc130	Fwd	GGTGAAAGGAAAGGCCAGAAC	21	59	52
	Rev	ATCGGTTGAGAGAGCCATGCT	21	60	52
Chfr	Fwd	AAGGAAGCCATGCCATATGC	20	59	50
	Rev	GGATCCTGCTCCCGTTCA	18	58	61
Ints1	Fwd	GGACTGCCTGCGATCCTTTA	20	59	55
	Rev	GCCCACGTACTTGTGGATGA	20	58	55
Pip4k2b	Fwd	CCGATTCTCAGCGTCCTGAT	20	59	55
	Rev	CATCCGGCATTAGCATGACA	20	59	50
Isg20l2	Fwd	CCAGGGAGACACATGCAGTAGAC	23	60	57
	Rev	CCTCTCCCCAGTGATTCTAGCTT	23	59	52
Ckm	Fwd	GCTGACCCCTGACCTCTACAATA	23	58	52
	Rev	CACCCCAGTCTGGATGACATC	21	59	57
Eno3	Fwd	GCAATTGCCTGCTCCTGAAG	20	60	55
	Rev	GTGCAAGTTTACAGGCCTGGAT	22	59	50
Serpinb2	Fwd	CAGGCACAAGCAGGAGATAAAA	22	58	45
	Rev	CCCCTGTGGTGTGTTGATTG	20	58	55
Hspb7	Fwd	AGCTGATGGCACAGTCATGAAC	22	59	50
	Rev	GCCGAGGTCACCGATGTT	18	58	61
Chac1	Fwd	GGTCATTGCCACACAGATCCT	21	58	52
	Rev	CTGCCAAACGCAATAAGTACTCA	23	58	43
Nrap	Fwd	GCTTTCTGTTAATAACTTCGTGAGTCA	27	58	37
	Rev	GACGCTAGTGAACGTGTTGTTCTT	24	59	46
Zp2	Fwd	TGACTGCTGGGCAACTTCTTC	21	59	52
	Rev	TCACAGCCATCCATGACAATCT	22	59	45
Atp1a2	Fwd	CATAAGGCTCAGGAGTCACAGAAG	24	58	50
	Rev	CACAGGGAGAAGTGGCAGCTA	21	59	57
Ctsd	Fwd	GGCGTCTTGCTGCTCATTCT	20	59	55
	Rev	ACTTGCGCAGAGGGATTCTG	20	59	55
Socs3	Fwd	GCCACCTGGACTCCTATGAGAA	22	59	55
	Rev	GGAGCATCATACTGATCCAGGAA	23	59	48
Cish2 (endogenous	Fwd	TCCAGATGTGCAAGGATAAACG	22	59	45
gene)	Rev	GGTTTGGTCAGGTACAGGTGAAC	23	58	52
Erk1	Fwd	CCTGCTGGACCGGATGTTA	19	58	58
	Rev	TGAGCCAGCGCTTCCTCTA	19	58	58
Epor	Fwd	TTGGTGTGTTTCTGGGAGGAA	21	59	48
	Rev	ACCCTCGAGCTGGTATGAGAAG	22	58	55
Hif1a	Fwd	TGCATCTCCATCTTCTACCCAAGT	24	60	46
	Rev	CCGACTGTGAGTGCCACTGTAT	22	59	55
Vegf	Fwd	GCAGGCTGCTGTAACGATGA	20	59	55
	Rev	CATGATCTGCATGGTGATGTTG	22	58	45

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ISSN: 1755-8794

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