Skeletal muscle alterations and exercise performance decrease in erythropoietin-deficient mice: a comparative study

Table 5 RT-qPCR results for 23 genes that were up- or down-regulated according to the microarray analysis

Detector	Ct Epo-d mice	Ct control mice	2-ddCt (fold change)	Microarray normalized ratio
Serpinb2	26.13	12.84	9.90E-05	0.57
Nrap	24.00	12.64	3.76E-04	0.63
Chfr	19.55	14.23	2.48E-02	1.71
Plcb2	20.42	16.01	4.66E-02	1.87
Chac1	15.50	11.66	6.93E-02	0.6
Ctsd	16.82	13.12	7.68E-02	0.66
Pip4k2b	15.07	12.43	0.16	1.70
Runx1	19.07	17.73	0.39	1.88
Eno3	13.69	12.71	0.51	0.53
Mcptl	13.48	12.63	0.55	3.25
Atp1a2	12.90	12.62	0.82	0.66
Hspb7	12.67	12.78	1.08	0.58
Socs3	15.95	16.60	1.55	0.88
Ccdc130	12.38	13.31	1.90	1.74
Isg20l2	12.45	13.61	2.22	1.70
Ckm	12.26	13.58	2.47	0.52
Ints1	18.37	19.98	3.03	1.71
Zp2	10.82	12.74	3.76	0.65
Erk1	14.33	24.29	982.97	1.11
Epor	13.83	12.85	0.50	NA
Cish2	12.89	12.90	1.00	NA
Vegf	13.04	13.89	1.79	NA
Hif1a	14.08	19.84	53.82	NA

The averaged Ct values of the triplicate analysis are quoted for EPO-deficient and control mice. The Ct values were normalized against Cish2 expression because the latter was not affected by the EPO deficiency. The relative expression of each gene in EPO-deficient mice was calculated by comparison with the corresponding gene expression in control mice. There was a statistically significant correlation (Pearson coefficient: 0.87; p < 0.01) between the expression values for 19 genes according to the RT-qPCR assays and the expression values according to the microarray analysis.

ISSN: 1755-8794