Table 1 List of proteins identified by mass spectrometry significantly changed between HCT116 gankyrin −/− and HCT116 cells
Spot No | Protein name | NCBI ID No. | Abbr nme | Mr (kD) | pl | Sequence Coverage (%) | Score | Change Fold | ||
|---|---|---|---|---|---|---|---|---|---|---|
Thero | Ober | Thero | Ober | |||||||
1 | Tumor protenin P53 Inducible protein 3 | AAC39528 | Pig3 | 34.4 | 40.2 | 5.9 | 6.0 | 10 | 115 | +1.9 |
2 | Cytochrome P450 family 1subfamily A | AAX62803 | CYP1A1 | 54.7 | 39.1 | 8.3 | 7.2 | 2 | 41 | +3.2 |
3 | Phosphatidylethanolamine binding protein | AAB32876 | PEBP1 | 20.9 | 24.9 | 7.4 | 8.0 | 50 | 347 | -5.6 |
4 | NEDD8-conjugating enzyme Ubc12 | NP_003960 | Ubc12 | 20.8 | 24.1 | 7.7 | 7.9 | 42 | 196 | -2.0 |
5 | 26S proteasome non-ATPase regulatory subunit 10 isoform 1 | NP_002805 | Gankyrin | 24.4 | 28.8 | 5.7 | 5.9 | 29 | 30 | -3.3 |
6 | S100-A9 | NP_002956 | S100-A9 | 13.2 | 15.0 | 7.4 | 4.2 | 33 | 149 | +2.1 |
7 | Protein kinase C inhibitor protein 1 | AAH51814 | YWHAZ | 35.3 | 30.1 | 4.5 | 4.2 | 39 | 347 | -9.8 |
8 | Proliferating cell nuclear antigen | 1AXC_C | PCNA | 28.7 | 38.2 | 4.4 | 3.8 | 44 | 229 | -9.1 |
9 | Tropomyosin alpha-4 chain isoform 2 | NP_003281 | TPM4 | 28.5 | 34.9 | 4.5 | 3.8 | 40 | 274 | -3.6 |
10 | Eukaryotic translation factor 6 isoform a | NP_002203 | p27BBP | 26.5 | 30.1 | 4.4 | 3.7 | 15 | 131 | -2.2 |
11 | Nucleoplasmin-3 | NP_008924 | NPM3 | 19.3 | 27.0 | 4.4 | 3.7 | 14 | 182 | -3.0 |
12 | Peptidyl prolyl cis trans isomerase A | NP_066953 | PPIA | 17.8 | 17.5 | 7.7 | 3.4 | 33 | 54 | -5.0 |
13 | Calcium/calmodulin-dependent protein kinase II gamma | 1J7O_A | CAMK2G | 8.4 | 17.5 | 3.79 | 3.48 | 39 | 107 | -2.9 |
14 | L-lactate dehydrogenase B chain | NP_002291 | LDHB | 36.6 | 45.3 | 5.6 | 5.2 | 8 | 96 | +5.2 |
15 | G protein beta subunit | AAA35922 | GNB2 | 37.3 | 45.1 | 5.7 | 5.2 | 23 | 218 | +6.3 |
16 | Proteasome subunit beta type-7 | NP_002790 | PSMB7 | 29.9 | 30.1 | 7.6 | 5.5 | 5 | 91 | -26 |
17 | Prosome beta subunit | AAB31085 | PSME2 | 25.8 | 29.8 | 5.6 | 5.4 | 28 | 175 | -1.6 |
18 | Proteasome subunit beta type-4 | NP_002787 | PSMB4 | 29.2 | 29.6 | 5.6 | 5.4 | 28 | 188 | -2.3 |
19 | Annexin A2 isoform 2 | NP_004030 | ANXA2 | 38.6 | 38.2 | 7.8 | 7.1 | 51 | 366 | -10.5 |
20 | Cofilin-1 | NP_005498 | Cofilin-1 | 18.5 | 18.9 | 8.2 | 8.4 | 50 | 347 | -2.6 |
21 | Chain K, Acetyl-Cypa:cyclosporine Comples | 2X2C_K | PPIA | 18.0 | 18.0 | 7.2 | 8.1 | 89 | 1473 | -3.0 |