Figure 5

miRNA up-regulation in bladder cell lines. miRNA molecules were used to up-regulate the levels of miR-27a, miR-138, miR-296-5p, miR-642, miR-886-3p and miR-944 in the bladder cell lines UMUC9, UMUC14, SLT4, 253JBV, RT4, CLR2169, HT1197 and 575A. miRNAs molecules were reverse transfected at 40 nM (n = 8) and after 48 h incubation culture media with or without cisplatin (GI50) added to the cells (n = 4). The viability of the cells was determined after 48 h incubation (96 h post transfection) by MTT-assay. LF2000 designate controls using the transfection reagent alone. A: Relative cell viability expressed as the viability of the miRNA transfected cells normalized to the negative control molecule for each cell line. B: Relative cell viability expressed as the viability of the antagomiR transfected cells treated with cisplatin (GI50), compared to the antagomiR transfected cells without cisplatin, normalized to the negative control molecule for each cell line. (#: the high reduction of cell viability imposed by the precursor miRs prohibits precise measure of effect of cisplatin treatment during miR down regulation).