Figure 6

miR-146a alters the expression of type 1 IFN inducible genes in C2C12 muscle cells. C2C12 cells were transfected with miR-146a mimics and miR-146a inhibitors, as well as the appropriate scrambled controls. 24 hours after transfection, cells were stimulated with mouse IFN-alpha for 4 hours before RNA was isolated and profiled for alterations in ISGs. Regulation of the IFN pathway was demonstrated by increased ISG transcript levels following inhibition of miR-146a, as well as decreased ISG transcript levels following addition of miR-146a. Values are shown as mean ± SEM and represent results from 3 independent experiments.