Table 1 Differentially expressed proteins in mitochondria of metastatic 5-8F compared to non-metastatic 6-10B cells, identified by 2D-DIGE and MS (Ratio = 5-8F/6-10B; +, up-regulated; -, down-regulated)
From: Mitochondrial proteomics of nasopharyngeal carcinoma metastasis
Proteins names | Spots no. | Accession number | Gene names | Subcellular location | Mass (Da) | pI | Mascot score | NMP | NUP | MOI | Sequence coverage (%) | Average ratio |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
1. Oxidoreductase Proteins | ||||||||||||
Peroxiredoxin 3 | 2 | gi|119569781 | PRDX3 | Mitochondria | 24,941 | 5.73 | 90 | 5 | 9 | PMF | 43 | +2.2 |
Peroxiredoxin 6 | 10 | gi|4758638 | PRDX6 | Mitochondria, lysosome | 25,133 | 8.36 | 71 | 5 | 8 | PMF | 21 | +2.5 |
Superoxide dismutase | 13 | gi|67782309 | SOD2 | Mitochondria | 20,882 | 8.36 | 71 | 4 | 4 | PMF | 52 | +3.3 |
[Mn], mitochondrial isoform B precursor | Â | Â | Â | Â | Â | Â | 62 | 2 | \ | MS/MS | 8 | Â |
Isocitrate dehydrogenase | 12 | gi|5031777 | IDH3B | Mitochondria | 40,022 | 6.47 | 75 | 6 | 10 | PMF | 15 | -3.3 |
[NAD] subunit alpha, mitochondrial precursor | Â | Â | Â | Â | Â | Â | 73 | 1 | \ | MS/MS | 3 | Â |
Protein disulfide isomerase related protein 5 | 5 | gi|1710248 | PDIA5 | Endoplasmic reticulum | 46,512 | 4.95 | 166 | 9 | 4 | PMF | 37 | +2.4 |
Cytochrome c oxidase subunit 5A, mitochondrial precursor | 9 | gi|190885499 | COX5A | Mitochondria | 16,923 | 6.30 | 72 | 4 | 3 | PMF | 38 | -4.4 |
Electron transfer | 6 | gi|2781202 | ETFA | Mitochondria | 33,418 | 6.95 | 73 | 4 | 2 | PMF | 27 | +2.6 |
flavoprotein | Â | Â | Â | Â | Â | Â | 95 | 3 | \ | MS/MS | 11 | Â |
2. Porin Proteins | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â |
Volatage-dependent anion selective channel protein 1 | 4 | gi|4507879 | VDAC1 | Mitochondria | 30,868 | 8.62 | 117 | 7 | 8 | PMF | 38 | +2.4 |
Volatage-dependent anion channel 2 | 1 | gi|55664661 | VDAC2 | Mitochondria | 30,842 | 8.00 | 101 | 6 | 5 | PMF | 34 | +2.2 |
3. Metabolism and regulation Proteins | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â |
Delta(3.5)-Delta(2.4)-dienovl-CoA isomerase, mitochondrial precursor | 11 | gi|70995211 | ECH1 | Mitochondria | 36,136 | 8.16 | 105 | 7 | 10 | PMF | 44 | +4.1 |
Prohibitin | 7 | gi|4505773 | PHB | Mitochondria | 29.843 | 5.57 | 158 | 8 | 1 | PMF | 44 | +2.0 |
Maspin | 15 | gi|56554671 | SERPINB5 | Extracellular region | 43,690 | 5.99 | 225 | 5 | \ | MS/MS | 15 | -3.2 |
Eukaryotic initiation factor 5A | 14 | gi|4261795 | EIF5A | Endoplasmic reticulum | 17,018 | 4.85 | 68 | 3 | \ | MS/MS | 15 | +3.2 |
26S protease regulatory subunit 7 isoform 1 | 3 | gi|4506209 | PSMC4 | Mitochondria, proteasome | 49,002 | 5.71 | 92 | 6 | 2 | PMF | 19 | +2.0 |
Ribosomal protein L7/L12, mitochondria | 8 | gi|1313962 | MRPL12 | Mitochindria | 21,593 | 9.04 | 86 | 4 | \ | MS/MS | 19 | -3.9 |
Calumenin isoform c precursor | 16 | gi|314122177 | CALU | Endoplasmic reticulum | 38,141 | 4.47 | 124 | 8 | 9 | PMF | 31 | +2.9 |