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Table 2 The methylation profiles of 8 imprinted genes by bisulphite PCR Luminex.

From: High-throughput detection of aberrant imprint methylation in the ovarian cancer by the bisulphite PCR-Luminex method

Cell Histology H19 GTL2 ZDBF2 PEG1 LIT1 ZAC PEG3 SNRPN
lines   BPL COBRA BPL COBRA BPL COBRA BPL COBRA BPL COBRA BPL COBRA BPL COBRA BPL COBRA
   CpG
(9,16)
CpG10 CpG
(4,8)
CpG8 CpG
(1,4,5)
CpG4 CpG15 CpG12 CpG
(5,17,19)
CpG16 CpG8 CpG7 CpG20 CpG21 CpG19 CpG19
NC1 N 67.5 34.6 62.2 76.8 66.4 46.2 50.4 38.5 42.7 27.4 62.3 48.4 78.8 67.4 65.3 57.3
NC2 N 55.6 42.7 67.3 82.1 67.9 72.5 54.8 46.2 53.7 37.5 65.2 69.3 65.4 48.9 78.5 67.3
NC3 N 65.8 58.3 81.2 63.5 76.3 82.1 45.3 63.9 26.4 41.6 50.2 72.1 65.8 83.2 56.3 68.2
NC4 N 58.6 72.1 76.4 80.4 80.2 67.2 37.5 58.2 35.8 54.7 47.2 52.8 45.9 58.3 65.2 74.9
OC1 S 10.32 95.4 69.8 53.2 87.3 92.4 83.1 59.2 64.3 48.3 89.5 98.3 74.4 83.2 65.4 58.4
OC2 M 16.8 19.4 61.4 70.5 95.7 97.2 48.8 62.2 62.1 48.0 20.1 10.2 92.1 89.5 29.1 49.7
OC3 S 87.4 93.2 96.3 93.4 83.7 28.6 98.3 90.4 87.4 56.9 87.3 96.5 98.5 96.4 28.4 37.4
OC4 S 42.8 56.8 97.3 93.2 99.3 98.5 0.9 10.4 13.2 17.1 100.9 98.3 48.7 47.2 28.1 24.2
OC5 S 83.4 56.4 89.2 96.6 95.3 72.8 93.2 102.4 28.4 32.6 100.3 91.3 98.8 95.6 54.3 60.4
OC6 E 78.9 72.1 94.3 82.1 38.3 18.3 83.2 68.5 34.2 56.2 85.3 78.9 57.2 97.5 40.5 53.1
OC7 C 0.4 21.3 19.8 29.3 41.7 55.5 60.5 42.0 21.2 11.3 34.5 40.1 90.7 99.0 23.5 52.8
OC8 C 0.1 6.9 6.4 10.5 91.7 87.3 81.8 88.1 35.2 29.1 49.8 53.2 102.1 95.7 21.8 33.2
OC9 S 82.3 54.3 37.4 65.2 22.4 42.8 23.6 18.5 43.7 56.8 69.5 74.9 68.5 90.2 38.2 56.3
OC10 C 83.8 67.8 78.4 65.9 27.9 32.3 63.8 58.7 42.8 60.2 89.9 93.7 59.6 43.9 38.9 71.4
OC11 S 92.4 91.6 17.1 25.0 6.0 18.0 43.2 60.1 18.2 10.0 72.5 58.2 101.4 100.0 75.5 60.5
OC12 S 54.7 53.8 23.7 18.8 48.6 74.3 68.9 58.4 48.3 93.6 98.8 67.4 39.8 73.4 57.1 51.2
OC13 S 80.3 66.2 42.3 40.1 86.0 74.2 93.4 95.2 10.5 8.8 94.4 90.5 102.1 100.0 36.0 39.5
OC14 C 98.1 87.7 67.4 40.3 99.3 90.2 99.6 95.5 12.3 1.6 34.3 41.2 92.1 100.0 38.1 51.2
OC15 C 45.2 45.0 93.0 98.2 99.7 95.3 92.1 94.8 55.0 47.5 27.5 36.4 86.1 97.2 48.7 10.1
OC16 S 36.9 44.6 90.6 92.0 93.0 92.3 100.7 100.0 13.0 6.2 28.9 35.7 93.2 100.0 22.9 17.6
OC17 C 93.8 89.8 61.8 68.3 21.8 22.2 37.9 42.5 48.4 31.8 20.6 31.1 98.3 95.5 0.0 0.2
OC18 C 34.4 46.0 94.5 98.0 95.3 98.0 60.1 73.2 35.2 29.4 17.3 35.0 87.2 100.0 22.7 11.1
OC19 C 67 30.7 47.8 62.1 87.4 85.3 4.1 10.1 11.2 0.0 100.1 100.0 84.6 92.1 30.4 31.4
OC20 M 1.6 42.6 58.1 60.3 62.4 55.2 73.4 81.4 18.3 13.4 49.2 68.2 92.8 98.5 86.7 44.8
OC21 S 8.0 24.5 80.2 87.0 96.6 90.1 77.2 81.2 4.5 8.1 100.5 97.5 107.8 98.2 16.7 16.2
Normal ovarian surface tissues
NT1 (42) 57.5 76.2 43.4 51.2 37.2 65.4 51.4 38.6 49.1 42.3 53.3 41.2 42.1 40.2 521 47.8
NT2 (48) 27.6 25.0 23.6 21.6 46.1 34.5 20.9 34.4 23.1 12.2 5.1 32.3 21.3 20.6 22.1 33.3
NT3 (40) 61.5 31.8 49.9 60.0 50.4 45.0 31.0 42.8 17.1 10.9 48.8 29.4 21.0 33.6 24.3 34.8
NT4 (38) 28.9 25.0 28.4 29.6 46.1 39.3 33.4 47.8 28.8 17.7 67.4 46.1 45.6 33.8 80.5 29.0
NT5 (55) 24.2 26.9 27.2 18.5 33.2 29.0 30.9 48.1 25.1 17.6 10.7 44.5 75.5 40.0 32.3 31.3
NT6 (45) 25.9 22.1 32.2 20.5 46.4 62.5 60.0 40.4 24.1 17.3 25.2 23.4 44.2 69.0 77.4 40.9
NT7 (41) 34.6 24.6 38.0 19.9 49.9 37.4 27.3 46.2 27.7 24.5 21.0 63.1 49.7 53.7 25.6 24.5
  1. Letters in blue and black boldface represent LOH, LOI and MOI, respectively. The Luminex value indicates the average value of DNA methylation