High-throughput detection of aberrant imprint methylation in the ovarian cancer by the bisulphite PCR-Luminex method

Hiura, Hitoshi; Okae, Hiroaki; Kobayash, Hisato; Miyauchi, Naoko; Sato, Fumi; Sato, Akiko; Suzuki, Fumihiko; Nagase, Satoru; Sugawara, Junichi; Nakai, Kunihiko; Yaegashi, Nobuo; Arima, Takahiro

doi:10.1186/1755-8794-5-8

Table 2 The methylation profiles of 8 imprinted genes by bisulphite PCR Luminex.

From: High-throughput detection of aberrant imprint methylation in the ovarian cancer by the bisulphite PCR-Luminex method

Cell	Histology	H19		GTL2		ZDBF2		PEG1		LIT1		ZAC		PEG3		SNRPN
lines		BPL	COBRA	BPL	COBRA	BPL	COBRA	BPL	COBRA	BPL	COBRA	BPL	COBRA	BPL	COBRA	BPL	COBRA
		CpG (9,16)	CpG10	CpG (4,8)	CpG8	CpG (1,4,5)	CpG4	CpG15	CpG12	CpG (5,17,19)	CpG16	CpG8	CpG7	CpG20	CpG21	CpG19	CpG19
NC1	N	67.5	34.6	62.2	76.8	66.4	46.2	50.4	38.5	42.7	27.4	62.3	48.4	78.8	67.4	65.3	57.3
NC2	N	55.6	42.7	67.3	82.1	67.9	72.5	54.8	46.2	53.7	37.5	65.2	69.3	65.4	48.9	78.5	67.3
NC3	N	65.8	58.3	81.2	63.5	76.3	82.1	45.3	63.9	26.4	41.6	50.2	72.1	65.8	83.2	56.3	68.2
NC4	N	58.6	72.1	76.4	80.4	80.2	67.2	37.5	58.2	35.8	54.7	47.2	52.8	45.9	58.3	65.2	74.9
OC1	S	10.32	95.4	69.8	53.2	87.3	92.4	83.1	59.2	64.3	48.3	89.5	98.3	74.4	83.2	65.4	58.4
OC2	M	16.8	19.4	61.4	70.5	95.7	97.2	48.8	62.2	62.1	48.0	20.1	10.2	92.1	89.5	29.1	49.7
OC3	S	87.4	93.2	96.3	93.4	83.7	28.6	98.3	90.4	87.4	56.9	87.3	96.5	98.5	96.4	28.4	37.4
OC4	S	42.8	56.8	97.3	93.2	99.3	98.5	0.9	10.4	13.2	17.1	100.9	98.3	48.7	47.2	28.1	24.2
OC5	S	83.4	56.4	89.2	96.6	95.3	72.8	93.2	102.4	28.4	32.6	100.3	91.3	98.8	95.6	54.3	60.4
OC6	E	78.9	72.1	94.3	82.1	38.3	18.3	83.2	68.5	34.2	56.2	85.3	78.9	57.2	97.5	40.5	53.1
OC7	C	0.4	21.3	19.8	29.3	41.7	55.5	60.5	42.0	21.2	11.3	34.5	40.1	90.7	99.0	23.5	52.8
OC8	C	0.1	6.9	6.4	10.5	91.7	87.3	81.8	88.1	35.2	29.1	49.8	53.2	102.1	95.7	21.8	33.2
OC9	S	82.3	54.3	37.4	65.2	22.4	42.8	23.6	18.5	43.7	56.8	69.5	74.9	68.5	90.2	38.2	56.3
OC10	C	83.8	67.8	78.4	65.9	27.9	32.3	63.8	58.7	42.8	60.2	89.9	93.7	59.6	43.9	38.9	71.4
OC11	S	92.4	91.6	17.1	25.0	6.0	18.0	43.2	60.1	18.2	10.0	72.5	58.2	101.4	100.0	75.5	60.5
OC12	S	54.7	53.8	23.7	18.8	48.6	74.3	68.9	58.4	48.3	93.6	98.8	67.4	39.8	73.4	57.1	51.2
OC13	S	80.3	66.2	42.3	40.1	86.0	74.2	93.4	95.2	10.5	8.8	94.4	90.5	102.1	100.0	36.0	39.5
OC14	C	98.1	87.7	67.4	40.3	99.3	90.2	99.6	95.5	12.3	1.6	34.3	41.2	92.1	100.0	38.1	51.2
OC15	C	45.2	45.0	93.0	98.2	99.7	95.3	92.1	94.8	55.0	47.5	27.5	36.4	86.1	97.2	48.7	10.1
OC16	S	36.9	44.6	90.6	92.0	93.0	92.3	100.7	100.0	13.0	6.2	28.9	35.7	93.2	100.0	22.9	17.6
OC17	C	93.8	89.8	61.8	68.3	21.8	22.2	37.9	42.5	48.4	31.8	20.6	31.1	98.3	95.5	0.0	0.2
OC18	C	34.4	46.0	94.5	98.0	95.3	98.0	60.1	73.2	35.2	29.4	17.3	35.0	87.2	100.0	22.7	11.1
OC19	C	67	30.7	47.8	62.1	87.4	85.3	4.1	10.1	11.2	0.0	100.1	100.0	84.6	92.1	30.4	31.4
OC20	M	1.6	42.6	58.1	60.3	62.4	55.2	73.4	81.4	18.3	13.4	49.2	68.2	92.8	98.5	86.7	44.8
OC21	S	8.0	24.5	80.2	87.0	96.6	90.1	77.2	81.2	4.5	8.1	100.5	97.5	107.8	98.2	16.7	16.2
Normal ovarian surface tissues
NT1 (42)		57.5	76.2	43.4	51.2	37.2	65.4	51.4	38.6	49.1	42.3	53.3	41.2	42.1	40.2	521	47.8
NT2 (48)		27.6	25.0	23.6	21.6	46.1	34.5	20.9	34.4	23.1	12.2	5.1	32.3	21.3	20.6	22.1	33.3
NT3 (40)		61.5	31.8	49.9	60.0	50.4	45.0	31.0	42.8	17.1	10.9	48.8	29.4	21.0	33.6	24.3	34.8
NT4 (38)		28.9	25.0	28.4	29.6	46.1	39.3	33.4	47.8	28.8	17.7	67.4	46.1	45.6	33.8	80.5	29.0
NT5 (55)		24.2	26.9	27.2	18.5	33.2	29.0	30.9	48.1	25.1	17.6	10.7	44.5	75.5	40.0	32.3	31.3
NT6 (45)		25.9	22.1	32.2	20.5	46.4	62.5	60.0	40.4	24.1	17.3	25.2	23.4	44.2	69.0	77.4	40.9
NT7 (41)		34.6	24.6	38.0	19.9	49.9	37.4	27.3	46.2	27.7	24.5	21.0	63.1	49.7	53.7	25.6	24.5

Letters in blue and black boldface represent LOH, LOI and MOI, respectively. The Luminex value indicates the average value of DNA methylation

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