Figure 2

Gene expression evaluated by real-time RT-PCR. (A) The TaqMan® Fast Universal PCR Master Mix and TaqMan® Gene Expression Assays from Applied Biosystems were employed to determine expression of the following genes (with assay ID’s): AOX1 (Hs00154079_m1), DHRS2 (Hs01061576_m1), HIST1H2AC (Hs00374312_s1), ICAM4 (Hs00169941_m1), MAP2K6 (Hs00992389_m1), OSMR (Hs00384276_m1), TP53 (Hs99999147_m1), and RB1 (Hs01078075_m1) in SiHa, HeLa, HaCaT, and PHKs following CDV treatment for 72 h. White bars represent the relative expression values of the microarray data. Black bars represent average RT-PCR values (±SD), relative to untreated cells and normalized against β–actin, from three independent samples. (B) Relative expression levels of HPV16 E6 and E7 in SiHa cells following treatment with CDV for different times, relative to untreated SiHa cells and normalized against β–actin. The bars show average values (±SD) of at least three independent experiments. An absolute 2-fold change difference was considered as biologically significant and is indicated by dashed lines in the figures.