Median methylation levels for GATA4 in TOF patients and controls. (A) The schematic represents the distribution of the CpG site and CpG island in the GATA4 gene; (B) The sequence shown represents a 500 base pair fragment (positions -392 – 107) in the 5′-UTR of GATA4; (C) GATA4_M median methylation level. All of the values represent the median with the interquartile range. TSS, Transcription Start Sites; red vertical line, CpG sites; red thick bars, CpG islands; M,MassARRAY amplicon; The region between arrows, target amplicon. *p < 0.05, **p < 0.01, ***p < 0.001 (Mann–Whitney test).