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Table 5 Ct values of EPs by qPCR

From: Disease specific characteristics of fetal epigenetic markers for non-invasive prenatal testing of trisomy 21

EP Placenta Blood Pvalue CtMBDof cff-DNA (n = 20) Pvalue
Trisomy 21 (n = 11) Normal (n = 14) Pregnant (n = 16) Non-pregnant (n = 6)
EP6         
  Ctinput 22.3±0.4 23.4±1.5 23.1±2.3 22.3±0.1 0.364 29.4 ±1.6 0.021
CtMBD 20.9±0.9a,b 20.9±1.0c,d 29.3±1.1 29.1±2.3 <0.001
ΔCt 1.5±0.9a,b 2.5±0.9c,d −6.2±2.2 −6.7±2.4 <0.001
EP7        
  Ctinput 22.8±0.9 24.8±2.5 24.0±2.7 21.8±0.6 0.143 29.5±1.5#
CtMBD 20.6±0.6a,b 21.4±0.8c,d 29.3±0.9 27.4±1.7 <0.001
ΔCt 2.2±1.1a,b 3.4±2.2c,d −5.3±2.9 −5.6±1.2 <0.001
EP9        
  Ctinput 20.7±0.2 21.9±0.6 21.2±0.6 22.9±0.1 0.243 34.8±4.6
CtMBD 28.8±0.6 28.9±1.2 27.0±1.7 28.6±2.0 0.469
ΔCt −8.1±0.4 −7.1±0.8 −5.8±2.1 −5.6±2.1 0.421
  1. Data are presented as mean±standard deviation. Ctinput denotes the threshold cycle of the input portion without methylated DNA enrichment. CtMBD denotes the threshold cycle of the methylated DNA enriched portion. The delta (Δ) threshold cycle (Ct) value was calculated as ΔCt = Ctinput - CtMBD. The values were compared using robust one-way analysis of variance (ANOVA), the post hoc Tamhane’s T2 test for multiple comparisons. In post hoc analysis, statistical significance (P < 0.05) is presented as follows: aTrisomy 21 placenta versus pregnant women blood; bTrisomy 21 placenta versus non pregnant women blood; cNormal placenta versus pregnant women blood; dNormal placenta versus non pregnant women blood; EP6 versus EP9; #EP7 versus EP9.