Comparison of nonsynonymous and splice mutation frequency between primary tumors and metastases. Each point represents a somatic single-nucleotide variant (SNV). Allele frequency enrichment was tested using the Fishers’ exact test. Gray dot, shared mutations; orange, significantly enriched mutations in primary tumors; green, significantly enriched mutations in metastases; purple, primary tumor-specific mutations; blue, metastasis-specific mutations. SNVs in cancer genes or genes located in cancer-related pathways are labeled with the corresponding gene names. SNVs in each of the 4 cases show in plots A to D separately.