Figure 7

Identification of 91 genes co-expressed with TNFSRF9 in neutrophils. (A) Expression of TNFSRF9 across 10 cell types. Expression is normalized to that observed in normal human skin. Values in yellow denote TNFSRF9 detection frequency. (B) Procedure used to identify TNFSRF9-co-expressed genes in neutrophils. For genes expressed in both skin and neutrophils, we calculated the Spearman correlation coefficient between the expression pattern of each gene and that of TNFSRF9 (n = 426 neutrophil microarray samples). Genes were ranked based upon the estimated Spearman correlation coefficient (raised to the 7th power). To identify an appropriate cut-off for TNFSRF9-co-expressed genes, we located the “bend” in the curve of descending correlation coefficient estimates (i.e., the rank minimizing the length of the red line). (C) The gene most strongly associated with TNFSRF9 expression in neutrophils was VAV1. The scatterplot compares normalized expression values (log₂ scale) between TNFSRF9 and VAV1 in neutrophils. (D) Expression of 91 genes co-expressed with TNFRSF9 in neutrophils (n = 426 microarray samples). Microarray samples were ordered with TNFRSF9 expression descending from left to right. Gold regions outline the middle 50% of normalized expression values among the 91 genes. Blue regions outline the middle 80% of normalized expression values among the 91 genes. Expression of each gene was normalized to its baseline (average) expression across all 426 samples, such that positive values correspond to above-average expression and negative values correspond to below-average expression.