Unsupervised clustering of peptides which can be phosphorylated by Akt. Unsupervised clustering depicting differential phosphorylation of peptides of the PI3K/Akt pathway by cell lysates treated with different concentrations of MK—2206 and for different time intervals, as compared with untreated cells. Blue: logFC < 0, orange: logFC > 0. Different treatment options are shown in different shades of gray (from light to dark gray: 1 μM 5, 30, 180, 960, and 10 μM 180 minutes of treatment with MK-2206. Light-green: 143B, dark green: U-2 OS.