Identification of gene-based responses in human blood cells exposed to alpha particle radiation

Chauhan, Vinita; Howland, Matthew; Wilkins, Ruth

doi:10.1186/1755-8794-7-43

BMC Medical Genomics

Table 3 Validation of PBMC gene responses identified by microarray via qPCR

From: Identification of gene-based responses in human blood cells exposed to alpha particle radiation

Radiation type	0.5 Gy alpha				1.0 Gy alpha				1.5 Gy alpha
Technology	qPCR		MA		qPCR		MA		qPCR		MA
Symbol	FC	PV	FC	PV	FC	PV	FC	PV	FC	PV	FC	PV
All doses
TRIAP1	2.15	0.00	1.81	0.00	3.32	0.00	2.14	0.00	3.78	0.00	2.57	0.00
GADD45A	2.94	0.00	2.27	0.01	4.43	0.00	2.77	0.00	4.70	0.00	3.38	0.00
RPS27L	2.01	0.00	1.67	0.01	2.59	0.00	1.90	0.00	3.57	0.00	2.04	0.00
MAP4K4	1.51	0.01	1.40	0.05	1.79	0.00	1.54	0.00	1.90	0.00	1.69	0.00
TNFRSF10D	2.15	0.01	1.81	0.01	2.94	0.00	2.08	0.00	3.08	0.00	2.39	0.00
ASTN2	6.18	0.01	2.07	0.01	8.02	0.00	2.67	0.00	8.98	0.00	3.15	0.00
TNFRSF10B	2.28	0.01	2.02	0.01	3.06	0.00	2.32	0.00	3.13	0.00	2.72	0.00
TMEM30A	1.92	0.01	1.72	0.01	2.67	0.00	2.13	0.00	2.76	0.00	2.45	0.00
TP53INP1	1.56	0.01	1.53	0.04	1.83	0.00	1.81	0.00	1.88	0.00	2.02	0.00
BAX	2.42	0.01	2.31	0.00	3.17	0.00	2.88	0.00	3.89	0.00	3.23	0.00
FAS	1.89	0.01	1.79	0.00	2.61	0.00	2.06	0.00	2.94	0.00	2.23	0.00
DDB2	2.96	0.01	3.56	0.00	5.15	0.00	4.41	0.00	5.26	0.00	5.49	0.00
AEN	4.24	0.01	2.16	0.01	7.09	0.00	2.80	0.00	7.46	0.00	3.14	0.00
GLS2	1.94	0.01	1.87	0.01	2.30	0.00	2.37	0.00	2.46	0.00	2.62	0.00
CMBL	3.99	0.01	2.52	0.01	6.79	0.00	2.90	0.00	7.41	0.00	3.57	0.00
PHPT1	2.28	0.02	2.23	0.00	3.50	0.00	2.57	0.00	4.17	0.00	3.03	0.00
PCNA	2.49	0.02	2.02	0.00	3.64	0.00	2.44	0.00	4.09	0.00	2.88	0.00
ASCC3	2.14	0.03	1.84	0.00	3.18	0.00	2.22	0.00	3.28	0.00	2.37	0.00
PPM1D	1.58	0.05	1.63	0.01	2.14	0.00	1.81	0.00	2.17	0.00	2.11	0.00
XPC	1.62	0.05	1.81	0.01	2.20	0.00	2.12	0.00	2.00	0.01	2.31	0.00
IER5	2.09	0.05	1.82	0.01	2.71	0.00	2.10	0.00	3.09	0.00	2.41	0.00
TNFSF4	2.91	0.06	2.93	0.00	4.98	0.00	3.69	0.00	5.34	0.00	4.67	0.00
SESN1	1.40	0.06	1.90	0.00	2.25	0.00	2.14	0.00	1.98	0.01	2.44	0.00
FBXO22	1.40	0.08	1.39	0.03	1.97	0.01	1.56	0.00	2.17	0.00	1.65	0.00
PHLDA3	9.91	0.09	3.18	0.00	22.89	0.00	3.96	0.00	28.82	0.00	4.89	0.00
ACTA2	2.12	0.09	2.54	0.01	2.72	0.01	3.07	0.00	2.74	0.01	3.60	0.00
CCNG1	0.91	0.10	1.80	0.00	2.49	0.00	1.97	0.00	2.56	0.00	2.14	0.00
MAMDC4	2.54	0.14	2.06	0.04	3.18	0.02	2.82	0.00	3.52	0.01	3.23	0.00
APOBEC3H	2.38	0.55	2.52	0.04	3.68	0.08	3.20	0.00	4.49	0.02	3.91	0.00
Med/High doses
SAC3D1	1.47	0.01	1.26	0.24	1.74	0.00	1.29	0.04	1.91	0.01	1.33	0.01
PVT1	2.02	0.01	1.42	1.00	2.76	0.00	1.68	0.02	3.45	0.00	1.85	0.00
TP53TAP1	1.42	0.01	1.20	1.00	1.60	0.00	1.39	0.03	1.92	0.01	1.45	0.01
TNFSF8	2.40	0.01	1.92	0.08	4.01	0.00	2.33	0.00	3.80	0.00	2.61	0.00
TMPRSS7	4.04	0.01	1.24	1.00	4.85	0.01	1.53	0.00	6.74	0.00	1.52	0.00
ZNF79	1.74	0.02	1.39	0.47	2.07	0.00	1.74	0.00	2.34	0.00	1.95	0.00
SLC7A6	1.64	0.03	1.62	0.15	1.98	0.01	1.89	0.00	2.22	0.00	2.15	0.00
CDKN1A	2.41	0.03	2.30	0.11	3.87	0.00	2.94	0.00	5.05	0.00	3.58	0.00
MDM2	2.93	0.04	1.24	0.31	4.44	0.00	1.30	0.02	5.17	0.00	1.45	0.00
FDXR	6.46	0.06	1.47	0.85	13.07	0.00	1.76	0.01	14.43	0.00	1.88	0.00
DCP1B	1.68	0.06	1.17	1.00	2.00	0.00	1.45	0.01	2.33	0.00	1.51	0.00
BBC3	4.10	0.06	1.40	0.66	6.75	0.00	1.65	0.01	7.25	0.00	1.83	0.00
ISG20	0.75	0.06	-1.44	1.00	0.46	0.00	-1.92	0.00	0.46	0.00	-2.30	0.00
FAM20B	1.49	0.07	1.08	1.00	1.45	0.01	1.25	0.03	1.55	0.01	1.35	0.00
DRAM1	1.41	0.07	1.32	1.00	1.70	0.00	1.55	0.03	1.91	0.00	1.66	0.00
CCDC90B	2.18	0.07	1.20	0.64	1.54	0.07	1.29	0.02	2.89	0.00	1.35	0.00
POLH	1.72	0.08	1.39	0.24	2.37	0.00	1.60	0.00	2.10	0.01	1.92	0.00
GSS	1.19	0.08	1.15	1.00	1.34	0.01	1.29	0.03	1.60	0.00	1.34	0.00
PRKAB1	1.40	0.08	1.29	0.59	1.62	0.01	1.49	0.00	1.85	0.00	1.60	0.00
ARHGEF3	1.37	0.09	1.32	0.59	1.74	0.01	1.51	0.00	1.68	0.02	1.55	0.00
ZNF337	1.23	0.11	1.34	0.25	1.53	0.02	1.44	0.01	1.61	0.02	1.52	0.00
HIST1H4B	1.34	0.11	1.83	0.75	1.62	0.02	2.21	0.03	1.79	0.01	2.59	0.00
LAMC3	2.76	0.12	1.54	1.00	3.63	0.00	1.93	0.02	4.50	0.00	2.13	0.00
GDF15	1.84	0.14	1.49	1.00	2.40	0.01	1.98	0.03	2.96	0.05	2.26	0.00
ISCU	1.15	0.15	1.18	0.98	1.29	0.01	1.24	0.05	1.47	0.00	1.26	0.01
TRIM22	1.28	0.15	1.69	0.25	1.96	0.02	2.06	0.00	1.63	0.02	2.16	0.00
BTG3	1.11	0.23	1.30	0.21	1.69	0.00	1.41	0.00	1.70	0.01	1.54	0.00
NUDT15	1.22	0.23	1.21	0.24	1.48	0.02	1.31	0.00	1.47	0.03	1.38	0.00
RETSAT	1.25	0.27	1.21	1.00	1.16	0.56	1.38	0.03	1.19	0.29	1.45	0.00
TRIM32	1.32	0.27	1.25	0.45	1.10	0.15	1.34	0.02	1.58	0.04	1.37	0.00
FHL2	1.74	0.30	1.50	0.44	2.47	0.02	1.82	0.00	2.65	0.01	2.20	0.00
FAM127B	1.14	0.30	1.21	0.54	1.53	0.01	1.31	0.00	1.58	0.05	1.38	0.00
PCNXL2	1.23	0.37	1.19	1.00	1.18	0.52	1.33	0.01	1.34	0.15	1.40	0.00
E2F7	2.23	0.37	1.33	1.00	3.19	0.39	1.49	0.05	4.08	0.43	1.72	0.00
TOB1	1.02	0.40	1.23	0.69	1.40	0.02	1.38	0.00	1.40	0.03	1.47	0.00
ANKRA2	1.07	0.44	1.39	0.27	1.63	0.01	1.48	0.02	1.60	0.02	1.60	0.00
CD70	2.45	0.55	2.04	0.75	4.44	0.04	2.50	0.04	5.05	0.03	3.01	0.00
EDA2R	8.96	0.61	1.31	1.00	19.58	0.87	1.57	0.02	31.64	0.29	1.67	0.00
IGFBP4	1.19	0.68	1.19	1.00	1.61	0.06	1.45	0.01	1.63	0.05	1.52	0.00

A comparison of the gene expression fold change (FC) responses obtained using microarray technology (MA) and qPCR at the three doses (0.5-1.5 Gy) of α-particle radiation examined. Transcripts that were shown to be differentially expressed at all three doses and the medium (1.0 Gy) and high (1.5 Gy) dose were validated using qPCR. PV indicates the p-value obtained using either ANOVA or microarray statistics. The table is divided into two sections; the first section is comprised of genes that were shown to be significant using MA technology at all three doses and the second section are those genes that were shown to be significant using MA technology at the medium (1.0 Gy) and high (1.5 Gy) dose.

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ISSN: 1755-8794

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