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Table 3 Validation of PBMC gene responses identified by microarray via qPCR

From: Identification of gene-based responses in human blood cells exposed to alpha particle radiation

Radiation type 0.5 Gy alpha 1.0 Gy alpha 1.5 Gy alpha
Technology qPCR MA qPCR MA qPCR MA
Symbol FC PV FC PV FC PV FC PV FC PV FC PV
All doses             
TRIAP1 2.15 0.00 1.81 0.00 3.32 0.00 2.14 0.00 3.78 0.00 2.57 0.00
GADD45A 2.94 0.00 2.27 0.01 4.43 0.00 2.77 0.00 4.70 0.00 3.38 0.00
RPS27L 2.01 0.00 1.67 0.01 2.59 0.00 1.90 0.00 3.57 0.00 2.04 0.00
MAP4K4 1.51 0.01 1.40 0.05 1.79 0.00 1.54 0.00 1.90 0.00 1.69 0.00
TNFRSF10D 2.15 0.01 1.81 0.01 2.94 0.00 2.08 0.00 3.08 0.00 2.39 0.00
ASTN2 6.18 0.01 2.07 0.01 8.02 0.00 2.67 0.00 8.98 0.00 3.15 0.00
TNFRSF10B 2.28 0.01 2.02 0.01 3.06 0.00 2.32 0.00 3.13 0.00 2.72 0.00
TMEM30A 1.92 0.01 1.72 0.01 2.67 0.00 2.13 0.00 2.76 0.00 2.45 0.00
TP53INP1 1.56 0.01 1.53 0.04 1.83 0.00 1.81 0.00 1.88 0.00 2.02 0.00
BAX 2.42 0.01 2.31 0.00 3.17 0.00 2.88 0.00 3.89 0.00 3.23 0.00
FAS 1.89 0.01 1.79 0.00 2.61 0.00 2.06 0.00 2.94 0.00 2.23 0.00
DDB2 2.96 0.01 3.56 0.00 5.15 0.00 4.41 0.00 5.26 0.00 5.49 0.00
AEN 4.24 0.01 2.16 0.01 7.09 0.00 2.80 0.00 7.46 0.00 3.14 0.00
GLS2 1.94 0.01 1.87 0.01 2.30 0.00 2.37 0.00 2.46 0.00 2.62 0.00
CMBL 3.99 0.01 2.52 0.01 6.79 0.00 2.90 0.00 7.41 0.00 3.57 0.00
PHPT1 2.28 0.02 2.23 0.00 3.50 0.00 2.57 0.00 4.17 0.00 3.03 0.00
PCNA 2.49 0.02 2.02 0.00 3.64 0.00 2.44 0.00 4.09 0.00 2.88 0.00
ASCC3 2.14 0.03 1.84 0.00 3.18 0.00 2.22 0.00 3.28 0.00 2.37 0.00
PPM1D 1.58 0.05 1.63 0.01 2.14 0.00 1.81 0.00 2.17 0.00 2.11 0.00
XPC 1.62 0.05 1.81 0.01 2.20 0.00 2.12 0.00 2.00 0.01 2.31 0.00
IER5 2.09 0.05 1.82 0.01 2.71 0.00 2.10 0.00 3.09 0.00 2.41 0.00
TNFSF4 2.91 0.06 2.93 0.00 4.98 0.00 3.69 0.00 5.34 0.00 4.67 0.00
SESN1 1.40 0.06 1.90 0.00 2.25 0.00 2.14 0.00 1.98 0.01 2.44 0.00
FBXO22 1.40 0.08 1.39 0.03 1.97 0.01 1.56 0.00 2.17 0.00 1.65 0.00
PHLDA3 9.91 0.09 3.18 0.00 22.89 0.00 3.96 0.00 28.82 0.00 4.89 0.00
ACTA2 2.12 0.09 2.54 0.01 2.72 0.01 3.07 0.00 2.74 0.01 3.60 0.00
CCNG1 0.91 0.10 1.80 0.00 2.49 0.00 1.97 0.00 2.56 0.00 2.14 0.00
MAMDC4 2.54 0.14 2.06 0.04 3.18 0.02 2.82 0.00 3.52 0.01 3.23 0.00
APOBEC3H 2.38 0.55 2.52 0.04 3.68 0.08 3.20 0.00 4.49 0.02 3.91 0.00
Med/High doses             
SAC3D1 1.47 0.01 1.26 0.24 1.74 0.00 1.29 0.04 1.91 0.01 1.33 0.01
PVT1 2.02 0.01 1.42 1.00 2.76 0.00 1.68 0.02 3.45 0.00 1.85 0.00
TP53TAP1 1.42 0.01 1.20 1.00 1.60 0.00 1.39 0.03 1.92 0.01 1.45 0.01
TNFSF8 2.40 0.01 1.92 0.08 4.01 0.00 2.33 0.00 3.80 0.00 2.61 0.00
TMPRSS7 4.04 0.01 1.24 1.00 4.85 0.01 1.53 0.00 6.74 0.00 1.52 0.00
ZNF79 1.74 0.02 1.39 0.47 2.07 0.00 1.74 0.00 2.34 0.00 1.95 0.00
SLC7A6 1.64 0.03 1.62 0.15 1.98 0.01 1.89 0.00 2.22 0.00 2.15 0.00
CDKN1A 2.41 0.03 2.30 0.11 3.87 0.00 2.94 0.00 5.05 0.00 3.58 0.00
MDM2 2.93 0.04 1.24 0.31 4.44 0.00 1.30 0.02 5.17 0.00 1.45 0.00
FDXR 6.46 0.06 1.47 0.85 13.07 0.00 1.76 0.01 14.43 0.00 1.88 0.00
DCP1B 1.68 0.06 1.17 1.00 2.00 0.00 1.45 0.01 2.33 0.00 1.51 0.00
BBC3 4.10 0.06 1.40 0.66 6.75 0.00 1.65 0.01 7.25 0.00 1.83 0.00
ISG20 0.75 0.06 -1.44 1.00 0.46 0.00 -1.92 0.00 0.46 0.00 -2.30 0.00
FAM20B 1.49 0.07 1.08 1.00 1.45 0.01 1.25 0.03 1.55 0.01 1.35 0.00
DRAM1 1.41 0.07 1.32 1.00 1.70 0.00 1.55 0.03 1.91 0.00 1.66 0.00
CCDC90B 2.18 0.07 1.20 0.64 1.54 0.07 1.29 0.02 2.89 0.00 1.35 0.00
POLH 1.72 0.08 1.39 0.24 2.37 0.00 1.60 0.00 2.10 0.01 1.92 0.00
GSS 1.19 0.08 1.15 1.00 1.34 0.01 1.29 0.03 1.60 0.00 1.34 0.00
PRKAB1 1.40 0.08 1.29 0.59 1.62 0.01 1.49 0.00 1.85 0.00 1.60 0.00
ARHGEF3 1.37 0.09 1.32 0.59 1.74 0.01 1.51 0.00 1.68 0.02 1.55 0.00
ZNF337 1.23 0.11 1.34 0.25 1.53 0.02 1.44 0.01 1.61 0.02 1.52 0.00
HIST1H4B 1.34 0.11 1.83 0.75 1.62 0.02 2.21 0.03 1.79 0.01 2.59 0.00
LAMC3 2.76 0.12 1.54 1.00 3.63 0.00 1.93 0.02 4.50 0.00 2.13 0.00
GDF15 1.84 0.14 1.49 1.00 2.40 0.01 1.98 0.03 2.96 0.05 2.26 0.00
ISCU 1.15 0.15 1.18 0.98 1.29 0.01 1.24 0.05 1.47 0.00 1.26 0.01
TRIM22 1.28 0.15 1.69 0.25 1.96 0.02 2.06 0.00 1.63 0.02 2.16 0.00
BTG3 1.11 0.23 1.30 0.21 1.69 0.00 1.41 0.00 1.70 0.01 1.54 0.00
NUDT15 1.22 0.23 1.21 0.24 1.48 0.02 1.31 0.00 1.47 0.03 1.38 0.00
RETSAT 1.25 0.27 1.21 1.00 1.16 0.56 1.38 0.03 1.19 0.29 1.45 0.00
TRIM32 1.32 0.27 1.25 0.45 1.10 0.15 1.34 0.02 1.58 0.04 1.37 0.00
FHL2 1.74 0.30 1.50 0.44 2.47 0.02 1.82 0.00 2.65 0.01 2.20 0.00
FAM127B 1.14 0.30 1.21 0.54 1.53 0.01 1.31 0.00 1.58 0.05 1.38 0.00
PCNXL2 1.23 0.37 1.19 1.00 1.18 0.52 1.33 0.01 1.34 0.15 1.40 0.00
E2F7 2.23 0.37 1.33 1.00 3.19 0.39 1.49 0.05 4.08 0.43 1.72 0.00
TOB1 1.02 0.40 1.23 0.69 1.40 0.02 1.38 0.00 1.40 0.03 1.47 0.00
ANKRA2 1.07 0.44 1.39 0.27 1.63 0.01 1.48 0.02 1.60 0.02 1.60 0.00
CD70 2.45 0.55 2.04 0.75 4.44 0.04 2.50 0.04 5.05 0.03 3.01 0.00
EDA2R 8.96 0.61 1.31 1.00 19.58 0.87 1.57 0.02 31.64 0.29 1.67 0.00
IGFBP4 1.19 0.68 1.19 1.00 1.61 0.06 1.45 0.01 1.63 0.05 1.52 0.00
  1. A comparison of the gene expression fold change (FC) responses obtained using microarray technology (MA) and qPCR at the three doses (0.5-1.5 Gy) of α-particle radiation examined. Transcripts that were shown to be differentially expressed at all three doses and the medium (1.0 Gy) and high (1.5 Gy) dose were validated using qPCR. PV indicates the p-value obtained using either ANOVA or microarray statistics. The table is divided into two sections; the first section is comprised of genes that were shown to be significant using MA technology at all three doses and the second section are those genes that were shown to be significant using MA technology at the medium (1.0 Gy) and high (1.5 Gy) dose.