Overview of analysis: This flowchart presents a brief overview of the analysis and subsequent experiments performed. 1. Construction of the weighted gene Co-expression network Analysis and relationship to innominate artery atherosclerosis. 2. Relationships between modules and atherosclerosis were confirmed using independent and publically available gene expression datasets. 3. Ontology analysis was performed using DAVID and identified macrophages as a potential cell type for validation of the network. In-vitro experiments are performed to characterize module genes. 4. Causality analysis is performed and experiments using macrophages from gene targeted mice are used to validate predictions.