Schematic diagram for the biological and computational design and annotation of the H&M microarray. The whole array design and analysis includes three stages, as described in the Materials and Methods Section. There were 3.6k newly designed probes in step A and 7.1k newly designed probes in step B. All probes on H&M (Agilent GEO platform GPL10749) were optimally and extensively annotated for cross-species hybridization as shown in Dataset S1. The Agilent pre-annotated cross-species hybridization probes from step A are given in Datasets S2-3, and the processes used to design the new probes in version 1 were previously published .