In Silicocancer cell versus stroma cellularity index computed from species-specific human and mouse transcriptome of xenograft models: towards accurate stroma targeting therapy assessment

Table 1 Cross-species hybridization experiments for H&M array

Initial experiment**
Dual channel array	1			2
Exposed to RNA in red channel	1.5ug Hs* RNA (sample 1)			1.5ug Mm* RNA (sample 2)
Exposed to RNA in green channel	0.75ug Hs* RNA + 0.75 Mm*			0.75ug Hs* RNA + 0.75 Mm*
Array design	Agilent 2x105k arrays
Validation experiment
Single channel array	1	2	3	4	5	6
Exposed to RNA	human	human	human	mouse	mouse	mouse
Array design	46k Human probes and 46k mouse probes

*Hs: Homo Sapiens, Mm: Mus Musculus
**In the initial experiment, two human-mouse dual-species arrays were custom printed on Agilent 2x105k arrays. Each array contained only one species of universal mRNAs on the red channel (either human or mouse) and had a control sample containing equal quantities of universal human and mouse mRNAs on the green channel.

ISSN: 1755-8794