Figure 4

Spry2 -Cterminus promotes somite myogenesis. pCAB-Spry2-Cterm was overexpressed in epithelial somites by electroporation followed by harvesting embryos at different time points of incubation: (A) 6-hours, (B) 11-hours, (C, Ci) 24-hours and (D, Di) 48-hours. An increase of MyoD expression was detected (A-Di) which was associated with an increase in the somites size after incubation of 48-hours (D, Di). Arrows in (A, B, C & D) indicate the electroporated somites and arrowheads in (Ci, Di) indicate the electroporated myotomes and cells. Magnifications: 20x in (A, B, C & D), 200x in (Ci, Di).