Figure 2

Snapshots of selected g-FLUA2H features. A. Homepage interface of g-FLUA2H, which includes a brief description of the application and links to start an analysis, check job status and get more information from the help page. B. Web-interface to upload input sequence files, parameters to disable the removal of redundant sequences and to remove alignment positions with a high fraction of gaps (insertions or deletions), and an option to receive email notification when the job submission is complete. C. A sample output page, providing users with a dropdown menu listing all the nonamer positions to view motif data compared between the animal and human hosts of the virus. The selection of a given nonamer position presents users with a detailed information on the distinct nonamer sequences at the position for both hosts, such as their motif classifications and incidences (% occurrence), amino acid substitutions relative to the index, strain name of origin virus in animal and human hosts, host species of the animal host, and the geographical distribution of the human host. D. Options for advanced analyses on the results, such as multi-motif analysis and transmission selection where users can analyse for any combination of the diversity motifs and incidences between the hosts, and filter for positive (the incidence of the corresponding motif is greater in human host) or negative selections (incidence of motif is greater in animal host).