Figure 6

Manipulation of miR-21-5p expression alters response to chemoradiation in vitro . A) Quantification of colony formation assays for SW480 cell line transfected with miR-21-5p mimic or mimic control. B) Quantification of colony formation assays for HCT116 cell line transfected with miR-21-5p inhibitor or inhibitor control. Cells were grown under control conditions or submitted to CRT treatment, which consisted of 5 μM 5-FU and a single dose of 2Gy (A) or 1Gy (B) radiation. Graphs show relative absorbance of colonies stained with crystal violet, normalized to untreated controls. All values are presented as means ± s.d. relative to mimic or inhibitor controls from two independent experiments with quadruplicates. *P < 0.005 for Student’s t-test.