|
Method
|
Novex
|
PPS
|
AMPure
|
Control
|
|---|
|
Sample
|
A1-A3
|
A4-A7
|
A8-A10
|
AC
|
|---|
|
Amount
|
1ug
|
1ug
|
1ug
|
1ug
|
|
RIN
|
8.2
|
8.2
|
8.2
|
8.2
|
|
Average Quality
|
37
|
37
|
37
|
37
|
|
Raw Reads
|
8.840869
|
11.871091
|
9.152952
|
8.491022
|
|
Size (<15 nt)
|
0.40 %
|
0.15 %
|
2.12 %
|
2.12 %
|
|
Low Quality (Q <30)
|
1.56 %
|
1.50 %
|
1.20 %
|
1.21 %
|
|
Adapter-Adapter
|
0.05 %
|
0.03 %
|
0.15 %
|
0.53 %
|
|
RNAs >40 nt
|
1.12 %
|
2.09 %
|
21.73 %
|
18.66 %
|
|
Surviving Reads
|
96.87 %
|
96.24 %
|
74.78 %
|
77.47 %
|
|
Unmapped
|
1.31 %
|
1.50 %
|
2.00 %
|
1.89 %
|
|
Unique-Mapped
|
7.21 %
|
6.44 %
|
6.52 %
|
6.42 %
|
|
Multi-Mapped
|
91.47 %
|
92.06 %
|
91.47 %
|
91.69 %
|
|
miRNA
|
96.92 %
|
96.45 %
|
96.03 %
|
96.24 %
|
|
Other ncRNAs
|
0.42 %
|
0.46 %
|
0.49 %
|
0.48 %
|
|
Repeat
|
0.77 %
|
1.04 %
|
0.88 %
|
0.82 %
|
|
Coding Gene
|
0.05 %
|
0.04 %
|
0.04 %
|
0.04 %
|
|
Unknown
|
0.52 %
|
0.48 %
|
0.41 %
|
0.42 %
|
|
miRNA Count (≥1)
|
415
|
425
|
370
|
372
|
- Small RNA data analysis shows the percentage, composition and quality of reads from eleven libraries produced by our bioinformatics pipeline in order to test and compare three different small RNA library preparation methods
