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Table 2 Purification method

From: Biomarker discovery: quantification of microRNAs and other small non-coding RNAs using next generation sequencing

Method

Novex

PPS

AMPure

Control

Sample

A1-A3

A4-A7

A8-A10

AC

Amount

1ug

1ug

1ug

1ug

RIN

8.2

8.2

8.2

8.2

Average Quality

37

37

37

37

Raw Reads

8.840869

11.871091

9.152952

8.491022

Size (<15 nt)

0.40 %

0.15 %

2.12 %

2.12 %

Low Quality (Q <30)

1.56 %

1.50 %

1.20 %

1.21 %

Adapter-Adapter

0.05 %

0.03 %

0.15 %

0.53 %

RNAs >40 nt

1.12 %

2.09 %

21.73 %

18.66 %

Surviving Reads

96.87 %

96.24 %

74.78 %

77.47 %

Unmapped

1.31 %

1.50 %

2.00 %

1.89 %

Unique-Mapped

7.21 %

6.44 %

6.52 %

6.42 %

Multi-Mapped

91.47 %

92.06 %

91.47 %

91.69 %

miRNA

96.92 %

96.45 %

96.03 %

96.24 %

Other ncRNAs

0.42 %

0.46 %

0.49 %

0.48 %

Repeat

0.77 %

1.04 %

0.88 %

0.82 %

Coding Gene

0.05 %

0.04 %

0.04 %

0.04 %

Unknown

0.52 %

0.48 %

0.41 %

0.42 %

miRNA Count (≥1)

415

425

370

372

  1. Small RNA data analysis shows the percentage, composition and quality of reads from eleven libraries produced by our bioinformatics pipeline in order to test and compare three different small RNA library preparation methods