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Table 2 Purification method

From: Biomarker discovery: quantification of microRNAs and other small non-coding RNAs using next generation sequencing

Method Novex PPS AMPure Control
Sample A1-A3 A4-A7 A8-A10 AC
Amount 1ug 1ug 1ug 1ug
RIN 8.2 8.2 8.2 8.2
Average Quality 37 37 37 37
Raw Reads 8.840869 11.871091 9.152952 8.491022
Size (<15 nt) 0.40 % 0.15 % 2.12 % 2.12 %
Low Quality (Q <30) 1.56 % 1.50 % 1.20 % 1.21 %
Adapter-Adapter 0.05 % 0.03 % 0.15 % 0.53 %
RNAs >40 nt 1.12 % 2.09 % 21.73 % 18.66 %
Surviving Reads 96.87 % 96.24 % 74.78 % 77.47 %
Unmapped 1.31 % 1.50 % 2.00 % 1.89 %
Unique-Mapped 7.21 % 6.44 % 6.52 % 6.42 %
Multi-Mapped 91.47 % 92.06 % 91.47 % 91.69 %
miRNA 96.92 % 96.45 % 96.03 % 96.24 %
Other ncRNAs 0.42 % 0.46 % 0.49 % 0.48 %
Repeat 0.77 % 1.04 % 0.88 % 0.82 %
Coding Gene 0.05 % 0.04 % 0.04 % 0.04 %
Unknown 0.52 % 0.48 % 0.41 % 0.42 %
miRNA Count (≥1) 415 425 370 372
  1. Small RNA data analysis shows the percentage, composition and quality of reads from eleven libraries produced by our bioinformatics pipeline in order to test and compare three different small RNA library preparation methods