Fig. 5

Genes regulated by PTTG1. a Differentially expressed genes in WT and PTTG1^−/− HCT116 cells. Upper: heat map of 662 differentially expressed genes (FDR < 0.05) between WT and PTTG1^−/− HCT116 cells. Lower: 37 out of 40 genes were consistent between microarray and qPCR. Results of qPCR were calculated using the ∆∆Ct method with GAPDH as an internal control. b Signaling network analysis of 662 differentially expressed genes revealed a network involve p53 and CTNNB1. c PTTG1 regulates DKK1 expression. HCT116 cells were transfected with pcDNA3.1 and negative control siRNA (control), pcDNA3.1 and PTTG1 siRNA (siPTTG1), control siRNA and pcDNA3.1-PTTG1 (PTTG1), respectively. Left: FoxM1, PTTG1 and DKK1 mRNA levels were detected by real-time qPCR. Right: FoxM1, PTTG1 and DKK1 protein levels were detected by western blot. d FoxM1 regulates PTTG1 and DKK1 expression. HCT116 cells were transfected with pcDNA3.1 and negative control siRNA (control), pcDNA3.1 and FoxM1 siRNA (siFoxM1), control siRNA and pcDNA3.1-FoxM1 (FoxM1), respectively. Left: FoxM1, PTTG1 and DKK1 mRNA levels were detected by qPCR. Right: FoxM1, PTTG1 and DKK1 protein levels were detected by western blot. All qPCR were performed in triplicates and normalized to GAPDH. β-actin was used as an internal control for western blot. Results from three experiments were expressed as mean ± SD. Western blot experiments were repeated three times. Results of a representative experiment are shown. *p < 0.05, **p < 0.01, ***p < 0.001