Fig. 1
Increased susceptibility of p53KO mice to PR8 infection. p53WT and p53KO mice (n = 10 per group) were intranasally inoculated with a sublethal dose of PR8 virus. Clinical signs and weight loss were assessed daily for 16 days. Lungs of infected mice were collected 3 and 6 dpi for analysis of viral loads. a Weight loss analysis in PR8-infected mice. Results are percentages of mean weight loss relative to initial weight. b The survival rates of PR8-infected mice. c Viral loads were determined by serial titration of lung homogenates in 10-day-old embryonated SPF chicken eggs. The EID50 was calculated. d The expression of viral hemagglutinin (HA) in the lungs of infected mice was determined by qRT-PCR. Values are means ± SE of at least 4 mice. *, p < 0.05 as assessed by the Student’s t-test; dpi, days post-infection