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Fig. 7 | BMC Medical Genomics

Fig. 7

From: Histone methylation levels correlate with TGFBIp and extracellular matrix gene expression in normal and granular corneal dystrophy type 2 corneal fibroblasts

Fig. 7

MLL1 knockdown attenuated the TGFβ1-induced expression of TGFBIp and ECM-associated genes. a Wild-type and GCD2-homozygous corneal fibroblasts were infected with MLL1 or control shRNA lentivirus. After puromycin selection, MLL1, H3K4me3, TGFBIp, and actin protein levels were analyzed by western blot. b-d Wild-type and GCD2-homozygous corneal fibroblasts were infected with MLL1 or control shRNA lentivirus. After puromycin selection, infected cells were stimulated with TGFβ1 (5 ng/ml) for 8 h, and mRNA and protein levels of TGFBIp were analyzed by RT-qPCR (b) and western blot (c). mRNA levels of ECM-associated genes were analyzed by RT-qPCR (d). Gene expression was normalized to that of GAPDH (internal control), and results are expressed as fold stimulation over control shRNA-infected wild-type control (mean ± standard error (SE); **P < 0.01 vs. control, n = 3)

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BMC Medical Genomics

ISSN: 1755-8794

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