|
Targeted Gene/Site(s)
|
Primer Direction
|
Primer Sequence (5’ to 3’)
|
|---|
|
JRK (cg24634471 and cg10596483)
|
Out Forward
|
GTAGGCGGGTTGAGTATTGG
|
|
Out Reverse
|
CGACCTAAACCCCGAACTCC
|
|
In Forward
|
GTTTTGGTGATAGGAAGGTAGTATTGT
|
|
In Reverse
|
[Biotin]-AACTCCCCCCTACTCTCTCCATCTATA
|
|
Sequencing
|
GGAAGATAGTTTTGGGTTGA
|
|
SLC6A4 (cg20592995)
|
Out Forward
|
TTGGGGAAAGGAGGTTAAGG
|
|
Out Reverse
|
GCTCGCTAACGATCACGATT
|
|
In Forward
|
AAGTGATAGGTGGTTAGATGAT
|
|
In Reverse
|
[Biotin]-CCTTTCATTTCACATAAAACCCTTAATATA
|
|
Sequencing
|
TTTTTTATTTAAGTTTTTGAGAGT
|
- Outer and inner PCR primer sequences used for the bisulfite pyrosequencing assay
