Table 3 Bisulfite pyrosequencing primers
Targeted Gene/Site(s) | Primer Direction | Primer Sequence (5’ to 3’) |
|---|---|---|
JRK (cg24634471 and cg10596483) | Out Forward | GTAGGCGGGTTGAGTATTGG |
Out Reverse | CGACCTAAACCCCGAACTCC | |
In Forward | GTTTTGGTGATAGGAAGGTAGTATTGT | |
In Reverse | [Biotin]-AACTCCCCCCTACTCTCTCCATCTATA | |
Sequencing | GGAAGATAGTTTTGGGTTGA | |
SLC6A4 (cg20592995) | Out Forward | TTGGGGAAAGGAGGTTAAGG |
Out Reverse | GCTCGCTAACGATCACGATT | |
In Forward | AAGTGATAGGTGGTTAGATGAT | |
In Reverse | [Biotin]-CCTTTCATTTCACATAAAACCCTTAATATA | |
Sequencing | TTTTTTATTTAAGTTTTTGAGAGT |