Fig. 6

Scoring cryptic splice sites against canonical splice sites. Cassette-like cryptic exons have 5′ and 3′ splice sites that are recognised by the spliceosome under TDP-43 depletion. 5′ and 3′ splice site scores are plotted separately. Cryptic splice sites are shown in red, canonical splice sites are shown in blue. Random sequences with AG or GT consensus are plotted in grey. Cryptic extensions are the result of a cryptic splice site competing with the canonical splice site. 5′ extensions result from cryptic 3′ splice sites whereas 3′ extensions result from cryptic 5′ splice sites. Box plots show first quartile, median and third quartile with the notches representing the 95% confidence interval of the median. Whiskers represent the minimum and maximum values that fall within 1.5 times the interquartile range. Outliers are plotted as black dots. Cryptic splice sites are compared to canonical splice sites with paired t-tests. ^∗ : P < 0.05, ^∗∗ : P < 0.001