Fig. 2

Screen shots of variant reports and exploration (BRAF p.V600E) (a). Variant annotation and prioritizing with available drugs. The variant report includes mutation position, variant allele frequency (VAF), patient frequencies, and drugs. Drug table shows mutation-relevant drugs recommended from various resources such as authentic drugs in clinical usage, OncoKB drugs classified in 4 levels, and drugs reported in PubMed abstracts. Filters to show variants of specified properties only are located at left-side. b Needle plot of annotated mutations in BRAF gene. Both height and circle size represent the frequency of mutation at each location among the TCGA patient cohort (LUAD in this example). The location and type of mutations found in the patient of the report is indicated by up-triangle icons under the protein sequence bar that contains the protein domains. The bottom part is for zooming in specific area. c Reads alignment plot around the mutation point (±100 bp range) is shown in the web browser using IGV java script version