Fig. 1

EDC3 loss-of-function is associated with differential expression of ARE-containing RNAs. a Differential expression of genes coding for ARE-containing RNAs in patients’ lymphoblastoid cell line samples. Out of the 22,123 expressed genes, 15,144 were listed in the database and 2956 of them were classified as ARE-containing. Bars indicate the standard deviation of the log₂(fold change). b Expression of EDC3 measured by real-time PCR after transfecting SKNBE cells with three different siRNAs targeting EDC3 (siEDC3-1, siEDC3-2, siEDC3-3) as well as a negative control (siNC). In siNC samples, EDC3 expression is set to 1. Gene expression levels are calculated from CT values of each sample, normalized against the mean CT value of the endogenous controls ACTB, B2M, PO, and TBP. T1-T3 refer to three biological replicate experiments. Samples chosen for RNA-seq are marked with a red asterisk. Bars indicate the standard deviation calculated on four technical replicates performed for each assay. c Differential expression of genes coding for ARE-containing RNAs in SKNBE cell line samples. Out of the 16,321 expressed genes, 12,749 were listed in the ARE-database and 2579 of them were classified as ARE-containing. Bars indicate the standard deviation of the log₂(fold change)