Fig. 3

exoRNA-seq in CSF from normal healthy donors. Exosomal mRNAs in CSF from four normal healthy (NH) donors was analyzed by next-generation sequencing (exoRNA-seq). a To assess analytical variability, technical replicates of exoRNA-seq performed. Count per million mapped reads (CPM) of each gene was represented in log scale. r means pearson’s product-moment correlation coefficient. b Position coverage of sequence reads within overall transcript is shown. Classification of transcript bin is described based on the length of transcripts. c Commonly detected exosomal mRNAs in CSF from all four NH donors are visualized by Venn diagram. d Commonly detected 4580 genes in NH were compared among donors. CPM of each gene was represented in log scale. Pearson’s product-moment correlation coefficient was also described in each pairs of comparison. e Expression levels of exosomal mRNAs are shown in the scale of counts per million mapped reads (CPM)