Table 2 Sequence of primers and Taqman probes
Gene | Primer (5′-3′) | Product length |
|---|---|---|
Mitochondrial gene ND1 | ||
ND1_f | ccctaaaacccgccacatct | 65 |
ND1_r | gagcgatggtgagagctaaggt | |
ND1_probe | HEX-ccatcaccctctacatcaccgccc-BHQ1 | |
Nuclear gene P30 | ||
RPP30_f | gatttggacctgcgagcg | 62 |
RPP30_r | gcggctgtctccacaagt | |
RPP30_probe | FAM-ctgacctgaaggctct-BHQ1 | |
TNF-а | ||
TNFA_f | ccctcaacctcttctggctcaa | 97 |
TNFA_r | ccaggtttcgaagtggtggtct | |
RARRES2 (Chemerin) | ||
RARRES2_f | tgaggacccccacagcttct | 92 |
RARRES2_r | aggcaccacgcatctcagtg | |
ADIPOQ (Adiponectin) | ||
ADIPOQ_f | tccccaacatgcccattcgct | 97 |
ADIPOQ_r | agcccaggaatgttgcagtgga | |
B2M (Reference gene) | ||
B2M_f | cctgccgtgtgaaccatgtg | 70 |
B2M_r | gctgcttacatgtctcgatccca | |