Method | Experimental Design | Distribution Assumptions | P-value | Validation of method for single subject inference in original methods publications | ||
---|---|---|---|---|---|---|
Internal | External | |||||
Simulation | Biological Replicates or Gold Standard | Translation to diagnosis, prognosis & treatment | ||||
edgeR [21] | r | NB | ✓ | ✓a | ✗ | ✗ |
DESeq [22] | r | NB | ✓ | ✓a | ✗ | ✗ |
DESeq2 [23] | r | NB | ✓ | ✓a | ✗ | ✗ |
DEGseq [24] | r | B | ✓ | ✓a | ✗ | ✗ |
NOISeqa [15] | r/ss (as NOISeq-sim) | NP | ✓ | ✓a | ±b | ✗ |
Mixture Model [5] | ss | MM | ±c | ✓ | ✗ | ✗ |
iDEG [14] | ss | NB | ✗ | ✓a | ✗ | ✗ |