Fig. 8

Dysregulated miRNAs in CF inversely interact with relevant dysregulated immune system mediators. a & b MA-plot segregates dysregulated miRNAs in CF compared to HC (a) and in CF patients with severe and mild phenotype. b FC values (Log2-transformed) plotted against the mean expression values (Log2-transformed) for each signature. Upregulated miRNAs are represented in red, downregulated in blue, and those not meeting the cutoff criteria (ANOVA F-test, FDR < 0.05, |log2 FC| ≥ 2) in black. c & d Inverse expression correlation of miRNAs and dysregulated genes involved in significant dysregulated canonical immune pathways in CF (see Fig. 7) (c) Four genes (CSF3R, CXCL1, CXCL3, and IL1B) downregulated in CF compared to HC and involved in dysregulated pathways showed inverse expression patterns with six miRNAs (miR-1972, miR-1273 h-5p, miR-4512, miR-877-3p, miR-5585-3p, miR-1273d). d Two dysregulated genes (ATF3 and ITPR1) associated CF phenotype, and involved in a dysregulated immune pathway, showed inverse expression patterns with two miRNAs (miR-92a-3p and miR-1248)