Fig. 4

A copy number independent gene signature is prognostic for tumor grade and patient survival. a, Heatmap showing the relative gene expression of the copy number independent differentially expressed genes that are involved in pathways that negatively regulate cell proliferation in GliNS2 CSCs compared to CB660 NSCs. b, Graph showing the copy number independent (CI) gene signature score for glioma samples in data set GSE1993 stratified by histological grade. Grade I/II = pilocytic astrocytoma (n = 2) and diffuse astrocytoma (n = 5), Grade III = anaplastic astrocytoma (n = 19), and Grade IV = glioblastoma (n = 39). Bar, median; box 25th to 75th percentile; whiskers, minimum and maximum. P = 1E-3, ANOVA analysis. c, Kaplan-Meier plots showing that the CI gene signature score is prognostic for patient survival in four independent data sets. The median CI score was used as the cutoff to dichotomize patients into CI-Hi and CI-Lo groups with the number of patients in each group indicated in parentheses. Hazard ratios were calculated using a Cox regression model, and p-values were calculated by using log-rank tests to determine statistical differences between survival curves. Below each Kaplan-Meier plot is a table showing the number of patients at risk over time