Fig. 5
From: DNA methylation status of TBX20 in patients with tetralogy of Fallot
Sp1 transcription factor binding to the TBX20_M1 region. a Sp1 transcription factor overexpression in HEK 293āT cell lines; b EMSAs confirmed the binding of the Sp1 protein to the TBX20_M1 region. Lane 1 contains only the Bio-labelled probe (TBX20_-708ābp/āā684ābp) alone; Lane 2: Bio-labelled probe + Sp1 protein in HEK 293āT cells; Lane 3: Mut-Bio-labelled probe + Sp1 protein in HEK 293āT cells; Lane 4: Bio-labelled probe + unlabelled competitor WT probe +Sp1 protein in HEK 293āT cells; Lane 5: Bio-labelled probe + unlabelled competitor Mut probe +Sp1 protein in HEK 293āT cells; c EMSA and super-shift western blot analyses confirmed the Sp1 protein binding. The arrows indicate the complex of Bio-labelled TBX20 probe and Sp1 protein